bZIP Transcription Factor UvATF21 Mediates Vegetative Growth, Conidiation, Stress Tolerance and Is Required for Full Virulence of Rice False Smut Fungus Ustilaginoidea Virens

bZIP Transcription Factor UvATF21 Mediates Vegetative Growth, Conidiation, Stress Tolerance and Is Required for Full Virulence of Rice False Smut Fungus

SUPPLEMENTAL DATA

Fig. S1. Target knock-out strategy and transformants verification of.

A. PCR verification of knockout transformants using different primer pairs. The DNA samples were collected from mycelia which had been cultured in the PSB medium for 7 days. The fragments ofwere amplified as a control to test DNA quality. WT indicates the wild-type strain JS60-2. B. The schematic diagram of UvATF21 target strategy. The arrows show the primers in this study.

Fig. S2. Verification of thecomplemented transformants.

A. The complemented transformants were amplified using different primer pairs. The plasmid, Δand WT show the complemented vector containing inserting UvATF21 fragment, knockout mutant, and JS60-2 strain, respectively. B. The complemented strategy and primers using in the verification.

Fig. S3. Detection of UvATF21 expression in knock-out and complemented strains.

The transcription ofwas detected by semiquantitative RT-PCR, using the cDNA from wild-type strain JS60-2, the knockout and the complemented strains. The RNA samples were collected from mycelia which had been cultured in the PSB medium for 7 days.

Fig. S4. The germination of the conidia.

The conidia of the testing strains were spread on the WA (water agar) or PSA media plates, respectively. The germinations were measured after 12 h and 24 h.

Table S1. Primers used in this study.