Qingchang decoction retention enema may induce clinical and mucosal remission in left-sided ulcerative colitis:A case report

lNTRODUCTlON

Ulcerative colitis (UC) is a chronic autoimmune disease characterized by relapsing-remitting abdominal pain, diarrhea, mucopurulent discharge, and rectal bleeding. Its lesions mainly involve the mucous membrane and submucosa of the colon. UC can be divided into proctitis, rectal-sigmoid colitis and extensive colitis[1], corresponding to 14.8%, 26.4% and 25.0% of all UC cases, respectively[2]. Left-sided colitis refers to colitis involving the lower part of the descending colon. The proportion of left-sided colitis in China exceeds 50%. The precise pathogenesis of UC remains unknown; therefore, medical therapy to cure the disease is not yet available. Therapeutic drugs include sulfasalazine, mesalazine,corticosteroids, immunosuppressants, and biological agents[3]. However, not all patients benefit from basic mesalazine and amino-salicylic acid treatment for left-sided UC. We report herein a case of a 44-year-old man with left-sided UC in whom standard oral and topical doses of mesalazine failed but clinical remission and mucosal healing were eventually achieved with the Chinese formula Qingchang decoction (QCD) enema. This publication reports and discusses the effects of QCD enema treatment on left-sided UC.

The princess, his wife, although she had got her husband by a trick, found that she could not trick him, and so she never tried, but busied herself in teaching her children and scolding her maids

CASE PRESENTATlON

Chief complaints

A 44-year-old Chinese man presented to Longhua Hospital with a history of left-sided UC for more than 6 years. He received oral treatment with mesalazine at a dose of 3 g/d (occasionally 2 g/d due to forgetting to take the medicine). Bowel movements were maintained 2-3 times a day, with a small amount of blood and mucus in the stool less than half of the time. Mesalazine suppository therapy and glucocorticoid enema were also administered briefly during this period, but the symptoms were not relieved. In the previous 6 years, colonoscopy was repeated annually, with results revealing a Mayo endoscopic score of 2 (erosion of the rectum and sigmoid colon covered with purulent secretions).

History of present illness

The patient suffered from recurrent bloody mucous stool. After another colonoscopy was conducted at a local hospital on October 30, 2019 (Figure 1), which suggested a diagnosis of active left-sided UC, he was admitted to our department on November 09, 2019. He reported bowel movements twice a day;mild, dull pain in the left lower abdomen that was relieved after a bowel movement; relapsing mucous discharge; and rectal bleeding when he was fatigued. We observed his tongue to be red with light yellow fur and a thin pulse.

History of past illness

The patient had no significant previous medical history.

Although UC has no corresponding disease name in TCM, it has long been recorded throughout history and is well understood. According to the description of the clinical manifestations of “changpi”,“Xiali”, “chronic dysentery”, and “recurrent dysentery” in the medical documents of the previous dynasties, it is not difficult to connect these diseases with UC. According to TCM theory, UC is located in the large intestine, and the main pathogenesis lies in dampness and heat accumulation. The stagnation of qi and blood with dampness and heat thus leads to the formation of pus. Heat toxicity also burns intestinal collaterals, causing blood perfusion outside of the collaterals. These pathological processes induce symptoms such as abdominal pain, diarrhea, and mucopurulent bloody stools. In the mid-1980s, Gui-Tong Ma, a renowned doctor at Longhua Hospital, developed the Qingchang suppository (QCS), which was the first suppository for rectal administration composed of pure Chinese herbs in China. QCS exerts its effect of clearing heat and dampness, promoting blood circulation and removing blood stasis by targeting four major pathogenic factors, namely, dampness, heat, toxicity, and blood stasis.

Personal and family history

The patient had no significant family history.

Physical examination

The mechanism of QCS has also been fully investigated. Sun[9] used QCS and sulfasalazine suppository (SASP) to treat rats with dextran sulfate sodium-induced colitis and normal control rats.The levels of the proinflammatory factors TNF-α and IL-6 were suppressed with both treatments,suggesting that QCS has anti-inflammatory effects. Moreover, the production of vascular endothelial growth factor (VEGF), hypoxia-inducible factor (HIF)-1α, and inducible NO synthase (iNOS) was also inhibited in the QCS- and SASP-treated groups, suggesting that QCS can restrain colonic vascular permeability and promote epithelial integrity and colonic hypoxia. Lu[14] administered low,medium, and high doses of QCS and SASP to rats with trinitro-benzene-sulfonic acid (TNBS)-induced colitis and observed the expression of F-actin protein, which is a major component of the cytoskeleton,through immunofluorescence. The study revealed that QCS can improve F-actin protein content based on reduction after modeling, which suggested that it can reduce the damage of UC to the mucosal cytoskeleton and protect the integrity of the intestinal barrier. These studies support the notion that QCS can effectively alleviate colonic inflammation in UC.

Laboratory examinations

No sign of inflammation was found in the blood analysis, and the leukocyte level was low, at 3.55 × 10/L, with predominant neutrophils (67.1%) and normal hemoglobin and platelet counts. The prothrombin level, partial thromboplastin times, and d-dimer levels were normal. The serum C-reactive protein level was also within normal limits, and the erythrocyte sedimentation rate was 2 mm/h. The blood biochemistry and urine analyses were normal. The ECG results were also normal.

Imaging examinations

The case described herein demonstrates that the Chinese formula QCD retention enema can effectively reduce both the clinical and mucosal remission of left-sided UC, thus providing another effective therapy other than mesalazine. This finding provides a new understanding of the treatment of left-sided UC and a basis for further studies to determine the underlying mechanism of the QCD treatment effects.

FlNAL DlAGNOSlS

The patient was diagnosed with UC subtyped as left-sided colitis based on endoscopy of the rectum and colon (Figure 1) and histology of the biopsy. His condition was mild in severity with an active persistent phase. The relevant Traditional Chinese medicine (TCM) syndrome was spleen deficiency and the accumulation of dampness and heat.

TREATMENT

The authors have read the CARE Checklist (2016), and the manuscript was prepared and revised according to the CARE Checklist (2016).

OUTCOME AND FOLLOW-UP

The patient received a total of 12 wk of QCD retention enema treatment combined with oral mesalazine.After the second episode of QCD treatment, on August 07, 2020, a colonoscopy was performed again.Normal mucosa was seen in the sigmoid colon (Figure 2A), and relative healing with patchy hyperemia was detected in the rectum (Figure 2B). The endoscopic Mayo score was evaluated as 1, which indicated that the integrated treatment induced both clinical and endoscopic remission. Subsequently, oral mesalazine was continued at a dose of 2 g/d with a QCD retention enema twice a week. The patient was last followed up on September 28, 2021, and he had no abdominal pain, nor bloody stool with mucus.

By now we were standing9 in the long Holiday line in the Layaway Department, and I figure it was now or never. I lay down on the ground and began screaming, I want that telephone, over and over again. Weary Christmas shoppers looked as my mother calmly said, Becky, you better get up by the count of three or else. One…Two…Three.

The QCS (Z05170722) consists of(IN),,,and[9]. As the most essential component of QCS, IN is a commonly used Chinese herb to cure UC by reducing inflammation and rectal bleeding. A randomized, placebo-controlled trial conducted by Japanese researchers demonstrated that 8 wk of IN administration (0.5-2.0 g per day) induced a clinical response and mucosal healing in patients with active UC[10].IN includes indigo and indirubin molecules[11], which act as representative human aryl hydrocarbon receptor (AHR) ligands. AHR signaling stimulates the production of interleukin-22 in mucosal type 3 innate lymphocytes, thus inducing the production of tight junction molecules and antimicrobial peptides, which contribute to mucosal healing[12]. IN also has an anti-inflammatory role by downregulating proinflammatory factors such as IL-1α, IL-6, IL-1β, IL-8 and TNF-α[13].

DlSCUSSlON

As a subtype of inflammatory bowel disease (IBD), UC is characterized by chronic inflammation of the colonic mucosa and recurrent attack. The exact etiology and pathogenesis of UC remain unclear, and treatment options are limited. For long-standing UC, typically 8 or 10 years after disease onset, there is a defined risk of dysplasia and colorectal cancer caused by persistent large-scale colonic inflammation[4].A targeted therapy that focuses on endoscopic mucosal healing rather than mere symptom remission has emerged as a new preferred concept in the management approach of IBD[5]. Mucosal healing refers to the resolution of inflammatory changes (Mayo endoscopic subscore of 0 or 1)[5]. It is essential to manage UC in the remission phase with the minimum category or dose of medicine due to the limited choice of medications.

It was a leaf torn out of an old book--a book of poetry There s more of that over there! said the grocer I gave an old woman some coffee for the book

The guidelines recommend oral mesalazine at a dose of ≥ 2.4 g/d combined with an amino-salicylic acid preparation enema ≥ 1 g/d as a first-line treatment for mild to moderate left-sided UC[3]. The guidelines also indicate that the local effect of mesalazine is better than that of topical corticosteroids.However, not all patients benefit from this treatment. For instance, the study by Marteau[6] showed that the remission rate of UC treated with mesalazine at a dose of 4 g/d combined with 1 g/d enema was 64%. A randomized controlled trial (RCT) by Hartmann[7] used mesalazine or budesonide enema therapy for patients with mild to moderate left-sided UC. The proportion of these patients in whom endoscopic remission (defined as an endoscopic score ＜ 2) was achieved was 71.7% (76/106) and 68.0% (76/103), respectively. Bokemeyer[8] reported that 78.8%-84.4% of left-sided UC patients were assigned an endoscopic score of 0 or 1 (0 = normal or inactive disease; 1= mild disease, erythema,decreased vascular pattern, and mild friability) after 12 mo of oral mesalazine at a dose of 2 g/d. In conclusion, oral mesalazine and/or enema therapy is ineffective for approximately 20% to 30% of patients with left-sided UC. The guidelines[5] recommend using systemic corticosteroid therapy, but a large portion of patients in practice are unwilling to receive oral or intravenous corticosteroid therapy due to their side effects. For patients with poor outcomes with mesalazine treatment and who are unwilling to accept systemic corticosteroid treatment, TCM therapy is an effective option, which is also documented in China’s IBD guidelines[1].

Messengers were forthwith despatched to the garden house, and after awhile they returned bringing a negro whom they had discovered in a secret chamber181 underneath the throne of Mihr-afruz, dressed in a dress of honour, and surrounded with luxury

The troll said that he would always be welcome; he had served him faithfully for the three years they had agreed upon, and he could make no objections to his leaving now

Physical examination showed no obvious abnormalities, except for slight tenderness in the left lower abdomen.

The clinical effect of QCS has been fully investigated. However, clinical trials aiming to identify the effect of QCD enema have not yet been conducted. A previous multicenter RCT demonstrated that an efficacy rate of 91.49% 8 wks after the initiation of QCS treatment at a dose of 2 g/d was achieved in patients with mild to moderate proctitis compared with 87.23% in the SASP treatment group (＞ 0.05)[15]. Complete efficacy was defined as the basic disappearance of clinical symptoms and mild mucosal inflammation or the formation of pseudopolyps found by colonoscopy. The outcome of this study also demonstrated that QCS has more advantages in relieving symptoms such as abdominal pain and distention, tenesmus, mucopurulent stool, and burning anal pain. After a one-year follow-up of the QCS group (9.3%), the recurrence rate was significantly lower than that of the SASP group (26.83%). These results indicated that QCS is effective in inducing and maintaining clinical and mucosal remission.

Considering that the suppository application location is mainly limited to the rectum while an enema can reach the descending colon, a QCD enema was thus developed according to the components of QCS for the treatment of left-sided UC. In the case reported here, the patient with active left-sided UC suffered for 6 years after standard oral therapy with mesalazine and intermittent topical treatment.Based on oral mesalazine maintenance treatment, an additional QCD enema at a dose of 180 mL/d successfully induced endoscopic mucosal healing. Although this is a case report, it provides preliminary evidence for the treatment of active left-sided UC. In the future, we will continue to follow up with this patient and collect more cases of QCD treatment for UC to provide more evidence for its treatment effect in left-sided UC.

CONCLUSlON

Mild inflammation, characterized by hyperemia, edema, erosions and friability was detected in the sigmoid colon (Figure 1A) and rectum (Figure 1B), 20 cm from the anus. Normal mucosa was noted in the remaining colon.

Li PH interpreted and reviewed the literature; Tang Y contributed to manuscript drafting and revision; Wen HZ was the patient’s attending doctor and was responsible for revising the manuscript for important intellectual content; all authors issued final approval of the version to be submitted.

National Natural Science Foundation of China, No. 81703986.

Informed written consent was obtained from the patients for the publication of this report and any accompanying images.

The authors declare that they have no conflict of interest.

Treatment with QCD enema was administered to the patient at a dose of 180 mL once a night, combined with oral mesalazine at a dose of 3 g/d. Rectal bleeding ceased after 4 wk of treatment, and after 8 wk of treatment, the patient had one or two bowel movements per day, and no mucous or bloody discharge was observed. Due to the satisfactory effect, the dose of oral mesalazine was reduced to 2 g/d, and enema therapy was stopped without a consultation. At the end of June 2020, relapsing mucous and bloody discharge 3 to 4 times a day occurred. On June 22, 2020, the patient came to our ward for further treatment with QCD enema. The stool frequency was reduced to 2 to 3 times a day, with no mucus or blood present after 4 wk of treatment.

Gretel is not the only fairy tale sister to rescue her brother. Also read Brother and Sister and The Six Swans on SurLaLune to read tales in which sisters rescue their brothers from spells or death.

This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BYNC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is noncommercial. See:https://creativecommons.org/Licenses/by-nc/4.0/

The lion is a red transgressor113, so when he comes rise and do him reverence; take a cloth and rub the dust and earth from his face, then set the game you have taken before him, well cleansed114, and lay the hands of respect on your breast

China

Pei-Han Li 0000-0003-3084-7280; Yu Tang 0000-0003-3181-2987; Hong-Zhu Wen 0000-0002-6203-4869.

Chen YL

A

Since my retirement8, I have searched for the next passion that could fill the void that a life playing baseball creates when you are no longer putting on those spikes9. It is a daunting10 journey, and many players never find that closure or that next love. But they keep looking, even if other parts of their lives are crumbling11 behind them. Maybe that was part of the problem: searching. I found myself agreeing when I heard John Locke, the main character on Lost, say, I found it just like you find anything else, I stopped looking.

Chen YL