Combined sevoflurane-dexmedetomidine and nerve blockade on post-surgical serum oxidative stress biomarker levels in thyroid cancer patients

lNTRODUCTlON

Thyroid cancer commonly presents as a malignant tumor occurring in the thyroid follicular epithelium. The incidence of thyroid cancer is increasing annually[1]. Monocyte chemoattractant protein (MCP-1) is a chemoattractant protein involved in the body’s inflammatory response[2]. Glutathione peroxidase (GSH-Px), a decomposition enzyme, is considered a sensitive indicator of the human stress response[3]. Adrenocorticotrophic hormone (ACTH) and norepinephrine (NE) are both important indicators of oxidative stress[4]. Clinical radical thyroidectomy is typically performed under general anesthesia using tracheal intubation, but can cause reversible unconsciousness and an absence of pain sensation. Cervical plexus nerve block, a novel form of anesthesia, has the advantages of simple implementation and an effective analgesic effect. However, it is reportedly associated with fear and anxiety in patients due to factors such as the need for an unnatural body position. Zhang[5] found that inhaled sevoflurane combined with dexmedetomidine is rarely used in China. Therefore, this study investigated the use of sevoflurane-dexmedetomidine inhalation general anesthesia combined with the cervical plexus nerve block in comparison with conventional general anesthesia in 96 thyroid cancer patients admitted to our hospital. We subsequently compared the serum levels of MCP-1, GSH-Px, ACTH, and NE between the two groups.

MATERlALS AND METHODS

Participant recruitment

We recruited patients with a diagnosis of thyroid cancer who were admitted to hospital between January 2019 and December 2020. The inclusion criteria were:(1) the patient met all the diagnostic criteria for thyroid cancer[6]; (2) diagnosis of thyroid cancerpathological examination; and (3) the patient was scheduled to receive radical thyroidectomy in our hospital. The exclusion criteria were:(1) a history of allergic reaction to anesthesia; (2) use of an anticoagulant or antifibrinolytic medication within the preceding week; (3) severe coagulation dysfunction; (4) neurological diseases which would prevent the patient from cooperating with the treatment; and (5) an incomplete patient history. Ninety-six patients were recruited and were divided into two groups. The control group comprised 47 patients (22 men) with a mean age of 61.17 ± 5.98 (range 51-75) years. The pathological types were papillary carcinoma (= 28), follicular carcinoma (= 15), undifferentiated carcinoma (= 2) and myeloid carcinoma (= 2). The tumor-nodes-metastasis (TNM) classification of the patients was as follows:15 cases were stage III, 22 were stage II, and 10 were stage I. The experimental group comprised 49 patients (23 men), with a mean age of 61.02 ± 5.39 (range 51-75) years. The pathological types were papillary carcinoma (= 30), follicular carcinoma (= 15), undifferentiated carcinoma (= 3) and myeloid carcinoma (= 1). The TNM classification was:16 stage III, 21 stage II, and 12 stage I cases. The age, sex, pathological type, and TNM classification did not significantly differ between the two groups (＞ 0.05).

Charles Chuck Plumb1 was a U.S. Navy jet pilot in Vietnam. After 75 combat missions, his plane was destroyed by a surface-to-air missile. Plumb ejected and parachuted into enemy hands. He was captured and spent six years in a communist Vietnamese prison. He survived the ordeal2（） and now lectures on the lessons he learned from that experience.

Procedure

Patients in both groups received radical thyroidectomy and were fasted of food and water for 8 h before surgery. The patient received an intravenous line and their vital signs were monitored in the form of Bispectral index, blood pressure, oxygen saturation, heart rate, and electrocardiogram.

In this study, sevoflurane-dexmedetomidine general anesthesia combined with cervical plexus nerve block exhibited high effectiveness, together with a reduced inflammatory response and stress indicators. However, the number of patients in this study was small, so larger-scale studies are needed in the future.

The experimental group received general anesthesia by sevoflurane-dexmedetomidine inhalation combined with cervical plexus nerve block anesthesia. For cervical plexus nerve block anesthesia, a mixture of 1% lidocaine (H11022295, Shanxi Jinxin Shuanghe Pharmaceutical Co., LTD.) and 0.375% ropivacaine (H20060137, AstraZeneca Pharmaceutical Co., LTD.) was injected using an ultrasoundguided single needle method, 8 mL into the deep cervical plexus on the affected side and 4 mL into the superficial cervical plexus. Further, 1 μg/kg dexmedetomidine (Jiangsu Nhwa Pharmaceutical Co., LTD. National Drug Approval H20110085) was injected intravenously, and sevoflurane was inhaled continuously to induce a concentration of 5%-6%, and 0.6 mg/kg rocuronium was injected intravenously after the patient lost consciousness. Sevoflurane 1.5%-3% and dexmedetomidine 0.5 μg/kg were used to maintain an anesthesia depth and BIS of 40-60.

The control group received conventional general anesthesia in the form of midazolam (Jiangsu Nhwa Pharmaceutical Co., Ltd. H20143222) at 0.05-0.1 mg/kg and sufentanil (Yichang Humanwell Pharmaceutical Co., Ltd., H20054171) at 0.3-0.5 μg/kg, etomidate at 0.3-0.5 mg/kg, and Rocuronium at 0.6 mg/kg. Mechanical ventilation was provided with a tidal volume of 6-8 mL/kg, the breaths rate is 10-14 times per minute, and an inhalation-exhalation ratio of 1:2. The partial pressure of end-expiratory carbon dioxide was maintained at 35-45 mmHg. After intubation, propofol (4-6 mg/kg per hour), remifentanil (0.15-0.5 μg/kg per minute), and cis-atracurium (0.1 mg/kg per hour) were continuously administered until the end of operation, to maintain a BIS of 40-60.

Sevoflurane-dexmedetomidine complex inhalation general anesthesia combined with the cervical plexus nerve block can reduce the postoperative inflammatory response in patients undergoing radical thyroidectomy for thyroid cancer, while inhibiting the stress response associated with surgery and maintaining high anesthetic quality and safety.

If the patient’s heart rate fell below 50 beats per minute, 0.5 mg atropine was injected intravenously. If the systolic blood pressure fell below 80 mmHg, 10 mg ephedrine was injected intravenously. Postsurgically, the endotracheal tube was removed when the patient had the following vital signs:the respiratory rate reached 16 breaths per minute with a tidal volume of 6 mL/kg, and the patient could open their eyes on command and clench their first strongly.

Outcome measures

The serum levels of MCP-1 and GSH-Px levels were measured before and after surgery. The serum ACTH and NE levels were measured 1 and 12 h after surgery. BIS was recorded, as were any side effects of the anesthesia. All outcome measures were compared between the experimental and control groups.

Serum analysis

A 2.5-mL sample of fasting venous blood was taken from each patient before the surgery, immediately after the surgery, and again at 1 and 12 h after the surgery. High-speed centrifugal delamination was used to separate the serum and the separated serum was sent to the laboratory. MCP-1 was detectedsolid-phase sandwich enzyme-linked immunosorbent assay (ELISA) with the MCP - 1 ELISA kit (Shanghai Tongwei Industrial Co., LTD). GSH-px was detected using the GSH-PX ELISA kit (Wuhan Mercer Biotechnology Co., LTD). ACTH was detected using the ACTH ELISA kit (Shanghai Zhenke Biotechnology Co., Ltd). NE was detected using the double antibody sandwich method with the NE ELISA kit (Shanghai Enzyme-linked Biotechnology Co., LTD). The serum levels of MCP-1 and GSH-Px were the mean values detected after the operation. Serum ACTH and NE were measured in the samples retrieved at 1 and 12 h post-surgically.

BIS measurement

BIS was recorded before the operation, for 20 min during the operation, and at the point that the patient opened their eyes. A BIS score of 100 indicates a fully awake state, and 0 indicates the complete absence of electrical brain activity. This range is divided into the following states:85-100 represents awake, 65-85 represents a sedative state, 40-65 represents anesthesia inhibition, and ＜ 40 indicates the possibility of burst suppression.

Postoperative anesthesia side effects

The serum ACTH and NE levels in the experimental group were significantly lower than those in the control group at 1 and 12 h post-surgically (Table 2).

Statistical analysis

SPSS version 20.0 was used for the statistical analyses. Age, MCP-1, GSH-Px, ACTH, and NE were presented as means ± SD. An independent-samples-test was performed for between-group comparisons and a paired sample-test was performed for within-group comparisons. The rates of anesthesia and incidence of adverse reactions of anesthesia were expressed as the percentages, and atest was used to compare groups. Statistical significance was set at＜ 0.05.

RESULTS

Serum MCP-1 and GSH-Px before and after surgery

Before surgery, there was no significant difference in the serum GSH-Px or MCP-1 Levels between the groups (＞ 0.05). After surgery, compared with the control group, the serum MCP-1 was significantly lower and GSH-Px was significantly higher in the experimental group (Table 1).

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Serum ACTH and NE at 1 and 12 h after surgery

The occurrence of side effects such as headache, ataxia, and lethargy was recorded in detail by experienced nurses and compared between the two groups. The rate of toxicity and side effects = (incidence of headache + incidence of ataxia + incidence of somnolence)/total number of cases × 100%.

BIS

The BIS in the experimental group was significantly lower than that in the control group during surgery, but exhibited the opposite trend at the point when the patient opened their eyes (Table 3).

Toxicity and side effects

The incidence of adverse reactions in the experimental group was 10.20% (5/49), and in the control group was 12.76% (6/47); this difference was not significant (＞ 0.05), as shown in Table 4.

DlSCUSSlON

Routine thyroid surgery can be completed under cervical plexus block in clinical practice[7]; however, sometimes a more radical thyroidectomy is required. Radical surgical procedures can affect thyroid gland lobules and cervical lymph nodes[8,9]. Although general anesthesia commonly used in clinical practice can block the limbic system and the hypothalamic projection system, it cannot mediate the stress response during surgery[10,11]. If thyroid surgery is performed under nerve block, an inadequate level of blockade may occur. Similarly, the stress reaction caused by surgery is more severe, with patients often experiencing anxiety, panic, and other negative emotions. It is therefore extremely important to combine blockade with more effective anesthesia methods[12,13].

Severe stress reactions can increase the risk of complications and mortality during surgery. Sevoflurane is a highly effective anesthetic inhalant, is more stable to heat and strong acids, and exerts analgesic and muscle relaxation effects during anesthesia. Dexmedetomidine hydrochloride can stimulate the α receptors and vascular motor centers in the locus coeruleus region of the brainstem to suppress the sympathetic[14] response. MCP-1 is a chemokine that affects the recombination of human monocytes and the production of inflammatory cytokines. The expression of GSH-Px, a peroxidedecomposing enzyme, is indicative of oxidative stress[15]. The results of this study showed that, compared with that in the control group, serum MCP-1 in the experimental group was significantly decreased, and GSH-Px was significantly increased post-surgically. This suggests that sevofluranedexmedetomidine general anesthesia combined with cervical plexus blockade can reduce the inflammatory response and oxidative stress response associated with surgery.

Surgical trauma can cause changes in hormone secretion that persist after surgery. Thus, the stress response caused by surgery can be detected in the form of changes in ACTH and NE[16]. In this study, the serum ACTH and NE levels in the experimental group were significantly lower than those in the control group at 1 and 12 h post-surgically. Sevoflurane-dexmedetomidine may activate the α receptors in the solitonal nucleus postsynaptic membrane, thus inhibiting sympathetic excitation and reducing NE release elicited by activity at central and peripheral nerve endings, consequently inhibiting plasma catecholamines[17]. As such, sevoflurane-dexmedetomidine general anesthesia combined with cervical plexus block may reduce the inflammatory response and oxidative stress in patients. Studies in the USS indicate that the combined use of sevoflurane-dexmedetomidine acts quickly, with a markedly improved analgesic effect and high anesthesia effectiveness[18,19]. The results of this study are consistent with those findings, in that the BIS during surgery was significantly lower in the experimental group (＜ 0.05). Dexmedetomidine exerts little effect on the hemodynamics, as it binds to central α2 receptors while controlling sympathetic activity through the inhibition of α and β adrenoceptor control of vascular tension, and reducing adverse reactions. Meanwhile, sevoflurane also has the advantage of entailing fewer adverse reactions during anesthesia. This study showed that there was no significant difference in the incidence of adverse reactions between the two groups, although these results are inconsistent with previous studies[20]. This inconsistency may be related to the dosage used.

They belonged to her daughter; and surely no one who had such adaughter could be silly. The mother was like a fountain ofquestions; and the daughter, who listened but never spoke8, mighthave passed for the beautiful maid of the fountain. How charming shewas! She was a study for the sculptor to contemplate9, but not toconverse with; for she did not speak, or, at least, very seldom. Has the pope a great family? inquired the lady.

CONCLUSlON

Vasilissa ran to the yard, and behind her she heard the old witch shouting to the locks and the gate. The locks opened, the gate swung wide, and she ran out on to the lawn. The Baba Yaga seized from the wall one of the skulls with burning eyes and flung it after her. There, she howled, is the fire for thy stepmother s daughters. Take it. That is what they sent thee here for, and may they have joy of it!

ARTlCLE HlGHLlGHTS

Research background

All study participants provided informed consent prior to study enrollment.

26.Godmother:The godmother did not become a common and well-known character in the Cinderella tale until Perrault incorporated her into his version of the story. Other versions of Cinderella in different cultures often have the heroine receive assistance from the deceased mother. The fairy godmother versions are the best known in Western culture thanks to Perrault and later versions from Disney and other sources.

Research motivation

This paper discusses the effects of sevoflurane dexmedetomidine inhalation general anesthesia combined with the cervical plexus nerve block on the postsurgical serum oxidative stress biomarker levels in thyroid cancer patients.

Research objectives

This study aimed to investigate the influence of sevoflurane-dexmedetomidine and nerve block on the oxidative stress after thyroid cancer surgery.

Research methods

We recruited 96 patients with a diagnosis of thyroid cancer admitted to hospital between January 2019 and December 2020. The levels of serum oxidative stress biomarkers were compared between the experimental group (sevoflurane inhalation and dexmedetomidine combined with cervical plexus block) and the control group (conventional general anesthesia) before and after surgery. Bispectral index (BIS) and the incidence of anesthesia side effects were also compared between groups.

This boast cost him his life; for when he went home Niels followedhim, and cut his throat, intending to rob the murdered man of thegold, which did not exist

Research results

Then said the poor woman, From whence knowest thou that I was a queen? The white maiden answered, I am an angel sent by God, to watch over thee and thy child

Research conclusions

Sevoflurane-dexmedetomidine inhalation general anesthesia combined with cervical plexus nerve block can reduce the postoperative stress and inflammatory responses in thyroid cancer patients, while maintaining high anesthesia effectiveness and safety.

Research perspectives

Sevoflurane-dexmedetomidine complex inhalation general anesthesia combined with cervical plexus nerve block could reduce the postoperative inflammatory response in thyroid cancer patients undergoing radical thyroidectomy, while inhibiting the stress response associated with surgery and maintaining high anesthetic quality and safety.

Du D and Wang Q designed the study; Qiao Q, Guan Z and Gao YF collected the data; Du D and Qiao Q analyzed and interpreted the data; Du D wrote the article.

Then it suddenly dawned on me. God was speaking to me the entire time I was attempting to be still. I remembered the music playing as I d begun my quiet time. He sent a sparrow to lighten my life with song. He sent a gentle breeze. He sent a neighbor to let me know that I had a friend. He sent my sweetheart to offer sincere sentiments of love. He caused my heart to flutter to remind me of life. While I was trying to count my blessings, God was busy multiplying them.

Following surgery, monocyte chemotactic protein-1 Levels were significantly lower in the experimental group compared to the control group, whereas glutathione peroxidase was significantly higher than in the control group. Serum adrenocorticotropic hormone and norepinephrine were significantly lower in the experimental group compared to the control group at 1 and 12 h after the operation. BIS was significantly lower in the experimental group than the control group at 20 minutes into the operation, but the direction of the difference was reversed at eye opening. The incidence of side effects was 10.20% (5/49) and 12.76% (6/47) in the experimental and control groups, the difference being non-significant.

Clinical Research Award of the First Affiliated Hospital of Xi’an Jiaotong University, China, No. XJTU1AF-CRF-2017-009.

This study was approved by The First Affiliated Hospital of Xi’an Jiaotong University Ethics Committee.

The incidence of thyroid cancer is increasing annually. Clinical routine thyroid surgery can be administered under a cervical plexus block; however, it cannot mediate the stress response during the surgery. If thyroid surgery is performed under a nerve block, an inappropriate blockade level can sometimes occur. Similarly, the stress response caused by surgery is more serious. Therefore, it is important to combine block with more effective anesthesia methods.

People have different views about which are the best years of one s life. One common idea is that the teenage years form this happy period. The modern Irish novelist, Edna O Brien, is particularly sensitive to the views of young women, and perhaps one can judge whether she views teenage as necessarily the happiest time in a young girl s life from this description of Caithleen in The Girl with Green Eyes. Living in Dublin, Caithleen has fallen in love with Eugene Gaillard, and has been seeking every possible way of `just bumping into him . Now, unexpectedly, they meet in the street ... Coming down the stone steps from the bookshop I met him. I saw him in that instant before he saw me and I was so astonished that I almost ran away.

The authors declare that there is no conflict of interest to disclose.

Next morning, when he awoke, he began to wish himself over the moat; and the thought occurred to him that if only the red ox were there, and thirsty enough to drink up all the water in the moat, he might walk across it dry shod

All data relevant to the study have been included in the paper.

The authors have read the STROBE Statement - checklist of items, and the manuscript was prepared and revised according to the STROBE Statement - checklist of items.

This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BYNC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is noncommercial. See:https://creativecommons.org/Licenses/by-nc/4.0/

China

25.Minx: Today a minx is a seductive woman who uses her sex appeal to exploit men (WordNet). Obsolete59 defintions of the word are a she puppy and a mink60 or mink otter61 (Webster s 1990).Return to place in story.

Dan Du 0000-0001-7383-7387; Qiao Qiao 0000-0001-5040-9347; Zheng Guan 0000-0003-0237-9336; Yan-Feng Gao 0000-0003-4439-9220; Qiang Wang 0000-0002-3637-2063.

Wang JL

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Wang JL