劉艷 王彥夫 楊詩惠 潘娟 管偉 匡海學 楊炳友




摘要 目的:對洋金花正丁醇部位化學成分進行分離鑒定研究。方法:采用硅膠柱色譜,ODS柱色譜,硅膠薄層色譜,高效液相色譜法(HPLC)進行分離純化,結合1H-NMR、13C-NMR、MS等波譜學數據進行結構鑒定。結果:從洋金花藥材粗提液中提取到了11個化合物。分別鑒定為:生物堿類:(S)-(-)-2-phenylpropionitrile(1)、merobatzelladine A(2)、木蘭花堿(3)、crotonine(4)、cyclo(Phe-Leu)(5)、pyrrolezanthine(6)、(E,E)-N,N-dityramin-4,4′-dihydroxy-3,5′-dimethoxy-β,3′-bicinnamamide(7)、N1,N5,N10 tri-p-coumaroylspermidine(8)、7-hydroxy-1-(4-hydroxy-3-me-thoxyphenyl)-N2,N3-bis(4-hydroxyphenethyl)-6-methoxy-1,2-dihydronaphthalene-2,3-dicarboxa-mide(9)。其他類:p-hydroxyb-enzoyl p-coumaric acid anhydride(10)、methyl(Z)-9-octadeceno-ate(11)。結論:從茄科中首次分離得到11種化合物。
關鍵詞 洋金花;生物堿;化學成分;茄科;提取分離;結構鑒定
Isolation and Identification of Chemical Composition from Flos Daturae
LIU Yan,WANG Yanfu,YANG Shihui,PAN Juan,GUAN Wei,KUANG Haixue,YANG Bingyou
(Heilongjiang Univercity of Chinese Medicine,Harbin 150040,China)
Abstract Objective:Isolation and identification of chemical composition from the n-butanol fraction of Flos Daturae.Methods:Silica gel column chromatography,ODS column chromatography,silica gel thin layer chromatography and HPLC were used for separation and purification.The structure was identified by 1H-NMR,13C-NMR,MS and other spectral data.Results:A total of 11 compounds were extracted from the crude extract of Flos Daturae.They were identified as:Alkaloids:(S)-(-)-2-phenylpropionitrile(1),merobatzelladine A(2),magnof-lorine(3),crotonine(4),cyclo(Phe-Leu)(5),pyrrolezanthine(6),(E,E)-N,N-dityramin-4,4′-dihyd-roxy-3,5′-dimethoxy-β,3′-bicinnamamide(7),N1,N5,N10 tri-p-coumaroylspermidine(8),7-hy-dro-xy-1-(4-hydroxy-3-methoxyphenyl)-N2,N3-bis(4-hydroxyphenethyl)-6-methoxy-1,2-dihy-dronaphthalene-2,3-dicarboxamide(9).Other compounds:p-hydroxybenzoyl p-coumaric acid anhydri-de(10),methyl-(Z)-9-octadecenoate(11).Conclusion:The above 11 compounds were isolated from Solanaceae for the first time.
Keywords Flos Daturae; Alkaloids; Chemical constituents; Solanaceae; Extraction and separation;Structural identification
中圖分類號:R284.1文獻標識碼:Adoi:10.3969/j.issn.1673-7202.2022.03.012
洋金花為茄科植物白花曼陀羅Datura metel L.的干燥花,每年盛花期(4~9月)采摘,同時可在果實成熟期(5~10月)對洋金花的種子進行采摘。采摘后晾曬或低溫干燥得本品。洋金花為一年生草本植物,多生長于山坡草地或住宅附近[1]。主要分布于福建,江蘇、浙江,廣西,廣東,湖北,四川等地[2]。洋金花藥用歷史悠久,生理活性較強,始載于《本草綱目》,曰“曼陀羅生北土,人家亦栽之。八月開白花,凡六瓣,狀如牽牛花而大”。2020版《中華人民共和國藥典》記載其性味辛,溫,有毒;具平喘止咳,鎮痛解痙,麻醉之功效[3]。主治哮喘咳嗽、脘腹冷痛、風濕痹痛、小兒慢驚等病癥,并可作為外科手術的麻醉劑。洋金花提取物對B型單胺氧化酶抑制劑(MAO-B)有抑制作用,可逆性MAO-B在治療帕金森病(Parkinson Disease,PD)中具有其獨特優勢[4]。且洋金花在治療銀屑病有效部位具有較強的抗炎、抗皮膚瘙癢及抗過敏等藥理作用[5]。洋金花的主要化學成分包括醉茄內酯類、生物堿類、黃酮類等;已報道其具有顯著的抗過敏、抗腫瘤、抗炎等多種生物活性[6-8]。……