Study of Guanhuang Ganmao Keli on the antiviral activity on human respiratory syncytial virus

Yue Wu, Xu Yang, Tao Sun?, Tao Liu?

1. School of Traditional Chinese Medicine, Hainan Medical University, Haikou 571199, China

2. Qinhuangdao First Hospital, Department of Nuclear Medicine, Qinhuangdao 066000, China

ARTICLE INFO

Article history:Received 25 Jan 2022 Received in revised form 3 Feb 2022 Accepted 13 Feb 2022 Available online 28 Feb 2022

Keywords:Guanhuang Ganmao Keli HRSV Antiviral activity Significant antiviral effect

A BSTRACT Objective: To study the antiviral activity of Guanhuang Ganmao Keli on human respiratory syncytial virus (RSV) in vitro. Methods: The Guanhuang Ganmao Keli was dissolved in pure water and filtered by a 0.22 micron filter to get solution. Cyclopiazonic acid (CPA) was used as positive control. The toxicity of Guanhuang Ganmao Keli on Hep-2 cells was tested by cell counting kit 8 (CCK-8). The protective effect of Guanhuang Ganmao Keli on RSV was evaluated under the highest toxic concentration. Results: The TC50 and EC50 of Guanhuang Ganmao Keli is 0.647 mg/ml and 0.014 mg/ml, respectively. Guanhuang Ganmao Keli showed significant antiviral effect when added 0、2、4、6 and 8h post-infection. Conclusions:Guanhuang Ganmao Keli is an effective antiviral agent on RSV in vitro.

?Corresponding author: SUN Tao, Associate Professor, M. M.. E-mail: 444822696@qq.Com; LIU Tao, Professor, M. D.. E-mail: 13647549420@163.com.

E-mail: 444822696@qq. Com; LIU Tao, Professor, M. D. ; 13647549420@163.com.

Fund Project: Hainan Provincial Natural Science Foundation of China (No.818MS070)