基于16S rDNA測(cè)序檢測(cè)原發(fā)性膽汁性膽管炎患者腸道菌群的變化

王廣宇　　陳瀟瀟　　侯顯良

[關(guān)鍵詞] 原發(fā)性膽汁性膽管炎;16S rDNA;腸道菌群;高通量測(cè)序

[中圖分類號(hào)] R575.2? ? ? ? ? [文獻(xiàn)標(biāo)識(shí)碼] B? ? ? ? ? [文章編號(hào)] 1673-9701（2021）19-0139-05

Detecting changes of intestinal microflora in patients with primary biliary cholangitis based on 16S rDNA sequencing

WANG Guangyu1? ?CHEN Xiaoxiao2? ?HOU Xianliang1

1.Central Laboratory， Guangxi Health Commission Key Laboratory of Glucose and lipid Metabolism Disorders， the Second Affiliated Hospital of Guilin Medical University， Guilin? ?541199， China; 2.State Key Laboratory for Diagnosis and Treatment of Infectious Diseases， the First Affiliated Hospital， College of Medicine，Zhejiang University， Hangzou? ?310006， China

[Abstract] Objective To study the abundance and diversity of intestinal flora in Primary biliary cholangitis （PBC） patients. Methods Nine patients with primary biliary cholangitis addmitted in the First Affiliated Hospital of Zhejiang University Medical College from January 2018 to January 2019 were selected as research subjects. Another 8 healthy subjects were selected as healthy controls. The intestinal flora DNA was extracted， and the corresponding gene fragments of V3-V4 region were amplified by high-throughput 16S rDNA sequencing， and the database was constructed for sequencing and bioinformatics analysis. Results In Alpha diversity analysis， there were no significant differences in ACE index， Chao index， Simpson index and Shannon index between the two groups（P>0.05）. Through OTU clustering and species annotation， we found that there were statistical differences in the abundance of some flora between the two groups （P<0.05）. Compared with healthy control group（NC）， Cellulosilyticum， Holdemanella， Lachnospiraceae， Lactococcus and Morganella were decreased and Hungatella was significantly increased in PBC group. In addition， real-time quantitative PCR was used to detect the content of intestinal Escherichia coli in the PBC group and the NC group， and it was found that the content in the PBC group was significantly higher than that in the NC group （P<0.05）. Conclusion Compared with healthy control group（NC）， the primary biliary cholangitis（PBC）group shows changes in intestinal flora to a certain extent. Further analysis of changes in intestinal flora is expected to provide new ideas for the study of the occurrence and development of primary biliary cholangitis and the search for new sensitive microbial markers.