Skeletal muscle metastases of hepatocellular carcinoma: A case report and literature review

Qi Song, Xiao-Feng Sun, Xiao-Li Wu, Yi Dong, Le Wang

Qi Song, Xiao-Feng Sun, Xiao-Li Wu, Yi Dong, Le Wang, Department of Ultrasound, Bethune First Hospital of Jilin University, Changchun 130000, Jilin Province, China

Abstract BACKGROUND The metastasis of liver cancer to skeletal muscle is extremely rare compared to other sites. We herein report a case of rapidly developing skeletal metastases following liver transplantation due to primary liver cancer.CASE SUMMARY A 70-year-old male with underlying chronic hepatitis B virus infection was diagnosed with hepatocellular carcinoma (HCC), for which he underwent liver transplantation in 2014. Six years after receiving the transplant, pathological examination confirmed the presence of HCC without vascular invasion. He was admitted to the hospital with a rapidly growing mass on his right thigh.Ultrasound examination revealed a mixed echo mass in the lateral soft tissue of the middle part of the right femur. Magnetic resonance imaging showed heterogeneous iso-signal intensity on T1-weighted images and heterogeneous hyper-intensity on T2-weighted images compared to the surrounding muscles.Pathological examination of the ultrasound-guided needle biopsy specimen revealed that it was similar to the previously detected liver cancer; the diagnosis was metastasis of HCC. Surgical excision was performed. There were no other sites of metastasis, and the patient recovered well after surgery.CONCLUSION This report presents a rare case of skeletal metastasis following liver transplantation for HCC. The study suggests a possible role for skeletal muscle metastasis mechanisms, which should be the focus of future research.

Key Words: Hepatocellular carcinoma; Transplant; Skeletal muscle metastasis; Pathological; Case report

INTRODUCTION

More than half a million new cases of hepatocellular carcinoma (HCC) are diagnosed annually worldwide. In China, HCC is the fourth most common cancer in males after pulmonary, stomach, and esophagus cancers, and the sixth most common in females[1]. Extrahepatic HCC can occur in one of three ways: direct extension,hematogenous spread, or lymphatic invasion. Rupture of HCC may result in intraperitoneal implantation of tumor cells on peritoneal or omental surfaces.Reported HCC metastatic sites include lungs, lymph nodes, bone, and brain. Rare sites of metastasis include the rectum, spleen, diaphragm, duodenum, esophagus, pancreas,seminal vesicle, and the urinary bladder. Treatment is often based on the stage of a tumor[2]. In addition to chemotherapy, liver transplantation offers the advantage of radical treatment for HCC because it can remove the tumor and restore normal liver function. A rare but possible outcome of this modality is skeletal muscle metastasis(SMM), a malignant tumor.

In this study, we report a case of a 70-year-old patient with long-term SMM of primary liver cancer in the muscularis of the right thigh, detected 6 years after liver transplantation without any other organ metastases.

CASE PRESENTATION

Chief complaints

A 70-year-old male had a rapidly growing mass on his right thigh, with no pain,redness, and swelling observed on the surface of the mass.

History of present illness

The patient had underlying chronic hepatitis B virus infection, and was diagnosed with HCC. He had received a liver transplant for primary liver cancer 6 years prior.He was admitted to the hospital after finding a rapidly growing mass on his right thigh.

History of past illness

The patient underwent a liver transplant for primary liver cancer 6 years prior.

Personal and family history

The patient was retired, living with his son, and had no family history of hereditary disease.

Physical examination

The patient was hospitalized and his physical parameters were examined. The vital signs were as follows: Body temperature 36 °C, heart rate 85 beats/min, respiratory rate 16 breaths/min, blood pressure 130/85 mmHg, and oxygen saturation in room air 95 %. There were no apparent signs of cranial nerve dysfunction. Muscle tone, strength,and deep tendon reflexes were normal in the upper extremities, while low in the inferior extremities with more on the right side. Abdominal cutaneous reflexes were normal. The remaining physical examination was unremarkable.

Laboratory examinations

After re-sectioning the right lateral femoral soft tissue mass, the patient was examined intraoperatively (right thigh mass) with a circumscribed mass of 5 cm × 3 cm × 2 cm.The findings revealed that small muscle tissue was attached to the surrounding mass,and a section of the mass was light brown, solid, and multinodular.

Imaging examinations

During admission, physical examination showed an oval-shaped mass with a diameter of about 5 cm on the anterolateral part of the right thigh. It was hard to touch, had poor mobility, and was attached to the surrounding soft tissue. He had normal blood flow from the right lower limb with adequate movement; the skin temperature was normal, no abnormality was found in the sensory nerve examination, the physiological reflex was normal, and no abnormality was found in the rest of the review. The ultrasound examination revealed a mixed echo mass in the lateral soft tissue of the middle part of the right femur, with a size of approximately 5 cm × 2 cm × 3 cm, which had a clear boundary and regular shape. Figure 1A presents an ultrasound image of the muscularis in the right thigh in which the blood flow is indicated with undefined boundaries. Figure 1B depicts the arterial spectrum taken by color Doppler flow imaging. The ultrasound image showed that soft tissues covered the right side of the femur, the nature of which remains to be determined. Figure 2 presents circular abnormal signals in the middle part of the right femur near the lateral soft tissue,accompanied by surrounding soft tissue edema by magnetic resonance imaging (MRI).The images showed heterogeneous iso-signal intensity on T1WI and heterogeneous hyper-intensity on T2WI compared to the surrounding muscles, which had signal inhomogeneity. The size was about 4.5 cm × 1.4 cm × 3.1 cm; the boundary was slightly blurred, and there were a few pieces of long T2 signal in the adjacent soft tissue (Figure 2A and B).

FINAL DIAGNOSIS

The final diagnosis of skeletal metastases was made based on pathological results and clinical history, where the patient had liver transplantation for liver cancer without affecting any other organ metastases.

TREATMENT

The patient underwent mass and peripheral musculature resection of the right vastus lateralis.

OUTCOME AND FOLLOW-UP

After resectioning the right lateral femoral soft tissue mass, the patient was examined intraoperatively (right thigh mass) with a circumscribed mass of 5 cm × 3 cm × 2 cm.Small muscle tissue was attached to the surrounding mass, and a section of the mass was light brown, solid, and multinodular. Figure 3 shows the immunohistochemical results: cluster of differentiation 68 (-), GATA (-), Inhibin A (-), Ki-67 (+40%), prostatespecific antigen (-), vimentin (-) cytokeratin 20 (CK20) (-), CK7 (-), CK-pan (+), giant cell fibroma-3 (lesions+), hepatocyte paraffin 1 (-), S-100 (-), thyroid transcription factor-1 (-), villin (weak + in local parts). The pathological testing confirmed the diagnosis of a malignant tumor based on medical history and immunohistochemical analysis, suggesting metastatic HCC with tumor thrombus visible in the vessel. After 1 mo, the patient was discharged, and he had no other symptoms.

Figure 1 Imaging examinations. A: Two-dimensional ultrasound images show mixed echoes in the muscularis of the right thigh with unclear boundaries; B:Power Doppler activity revealed significantly increased flow within the tumor.

Figure 2 Magnetic resonance imaging. A: Coronal scan of the arrow points iso-signal of the tumor intensity on T1-weighted images; B: The arrow indicates high-signal intensity on T2-weighted images compared with the surrounding muscle.

Figure 3 Pathological analysis. A: Immunohistochemical staining examination of metastatic hepatic carcinoma hepatocyte paraffine 1 (Stain, 400 ×); B:Pathological examination of the tumor in the right thigh (Hematoxylin stain, 200 ×); C: Immunohistochemical staining examination of the tumor in the right thigh of metastatic hepatic carcinoma glypican-3 (Stain, 400 ×).

DISCUSSION

HCC is ranked sixth in incidence of malignancies and fourth in mortality rate. The World Health Organization has predicted a global mortality of more than 1 million from HCC by 2030[1]. In China, liver cancer is the second leading cause of cancerassociated deaths[2]. Liver transplantation offers the advantage of radical treatment for HCC, as it can remove the tumor and restore normal liver function. The occurrence SMM, a malignant tumor that often occurs in patients with advanced cancer and appears to be a sign of systemic hematogenous metastases, is rare after liver transplant[3,4]. Almusarhedet al[5] reported a case of solitary biceps muscle metastasis from breast cancer, 6 mo after postoperative chemotherapy combined with endocrine therapy. Extensive European autopsy has shown that skeletal muscle subclinical metastases are more common than generally believed, with an incidence ranging from 0.2% to 17.5%[6]. Researchers have found that most common tumors with muscle metastases come from the thyroid, esophagus, stomach, pancreas, colon, rectum,bladder, breast, ovary, and prostate[7-10]. However, the most common sites of muscle metastases are the erector spine, iliopsoas, and paravertebral muscle[11-13], which are rare in skeletal muscles of limbs. Guidiet al[14] reported a case of epithelial cell carcinoma in the bladder, metastasizing into the superficial flexor muscle of the thumb, while, Katafigiotiset al[15] reported two cases of sartorius metastatic tumors.Nevertheless, long-term SMM as described in this study, with onset after 6 years of liver transplantation has rarely been reported. Clinically, the disease is insidious and is generally detected by imaging, with only a few patients showing specific clinical symptoms. Studies have addressed the rare pathogenesis of SMM such as a report by Pergoliniet al[16]. They found that: skeletal muscle had an impact on tumor cell sedimentation due to mechanical muscle movement, instability of blood flow, and changes in tissue pressure; lactic acid in the muscle inhibits the proliferation of tumor cells; and pH was reduced in the microenvironment of the skeletal muscle[16]. Besides,muscle cells can secrete cytokines that inhibit tumor cell proliferation such as tumor necrosis factor, lymphocyte infiltration factor, and transforming growth factor. In a series of animal experiments, Luoet al[17] showed that skeletal muscles produced specific muscle-derived inhibitors that could inhibit the growth of tumor cells through autocrine or paracrine signaling. Magee and Rosenthal pointed out that local trauma could be one of the risk factors for metastasis due to dynamic imbalances between local physiological and mechanical factors[18]. Clinical symptoms or imaging examinations are often difficult to confirm the disease as there is a possibility of misdiagnosis of primary soft tissue tumor or soft tissue injury. Since early diagnosis and detection of skeletal muscle metastases are relevant for the treatment and prognosis, accurate diagnosis is essential. Commonly used clinical test methods include ultrasound, computed tomography, nuclear magnetism, and positron emission tomography computed tomography[19-23]. MRI is used for differential diagnosis and to analyze and compare the range of affected muscles to the surrounding muscles across several sections and angles. Liet al[24] performed MRI analysis on 31 patients with SMM. They found that most MRI images had heterogeneous iso-signals in T1W1,homogeneous high signals in T2W1, and heterogeneously enhanced signals in TT2W1.The soft tissues in the skeletal muscle need to be differentiated from the following diseases: Malignant fibrous histiocytoma, fibrosarcoma, rhabdomyosarcoma,schwannoma, chondroid lipoma,etc.Malignant fibrous histiocytoma, which is more common in middle-aged and older adults, is the most common type of soft tissue malignant tumor in the thigh. MRI shows equal or low signal in TIWl and high signal in TIW2. Fibrosarcoma is found in the deep soft tissue of the trunk and lower limbs of older adults, and the position is usually shallower than the surface. The MRI results indicate a low TIW1 signal, while TIW2 was high. Rhabdosarcoma typically occurs in children and adolescents, affecting the muscles of the limbs or trunk. It manifests itself as a fast-growing painful muscle mass. An MRI imaging shows TIWl and muscle signals, while TIW2 shows a high signal. Schwannoma referred to as a Schwann cell tumor is a condition in which any nerve around a larger nerve may be involved. Tinel is positive, and the MRI scan often shows fusiform masses along the direction of the peripheral nerve trunk. Chondroid lipoma is a benign adipose tissue tumor that exists as a slowly enlarging mass, mostly subcutaneous, with a partial intramuscular mass.Histology is composed of protocells, adipocytes, preadipocytes, mature adipocytes,and chondroid with features of embryonic fat and cartilage. Treatment for this disease is controversial as there is no exact treatment regimen addressing metastatic lesions located in the skeletal muscles. There is also no standard treatment strategy dealing with metastatic lesions without an intrahepatic component. Treatment modalities include a combination of surgery, chemotherapy, irradiation, and clinical practice. The prognosis of patients with SMM is extremely poor. Four cases died among the reported cases, with a median survival time range 6–18 mo. Only one patient, who underwent chemoradiotherapy for multiple skeletal metastases, remained alive at 24 mo after recurrence[25]; however, the underlying mechanisms remain poorly understood[26-29]. Skeletal muscle mass in the body accounts for a large percentage of overall body weight, and there is a significant amount of blood supply to the entire skeletal musculature of the body. Clinical and pathological data have shown that skeletal muscle tissue is resistant to the development of cancer metastases as muscular organs appear to be unsuitable for the growth of metastatic cells. Hence, the factors responsible for the rarity of skeletal muscle metastases are unknown. However,biomechanical destruction of cancer cells arrested in the skeletal muscle microvasculature is responsible for their rapid death[30]. When SMM is present, it is often suggested that the disease is in the terminal stage of blood-borne metastasis. A retrospective study by Plazaet al[31] observed that less than 2.5% of 174 SMM patients survived for more than 72 mo. No optimal treatment has been found so far for SMM due to its rarity, and the treatment of SMM lesions is limited to palliative treatment.Kimet al[32] proposed that surgical resection or external radiotherapy should be considered for isolated masses without other metastases to reduce pain and mass effect. However, Purkayasthaet al[33] reported a case of lung adenocarcinoma with left gluteus muscle metastasis in which acute metastasis of the lower back, bilateral supraspinatus muscle, and psoas muscle occurred after three cycles of personal computer chemotherapy and subsequent low-dose external radiotherapy. These clinical cases suggest that more clinical practice studies are needed for the diagnosis and treatment of SMM. The correct interpretation of the molecular and pathophysiological mechanisms of SMM may help design new therapies to combat the progression of the disease.

CONCLUSION

Here we report a case of an elderly male with SMM to his right thigh, 6 years after liver transplantation for liver cancer without metastases to other organs. HCC metastasis to the skeletal muscle is rare, not previously reported, and recognized as a possibility in patients with HCC.

SMMs have a poor prognosis that could cause functional impairment and increased short-term postoperative morbidity in patients with and without malignant diseases[34]. However, 1 mo later, this patient was discharged with no radiation or chemotherapy; we will continue to follow up for further analysis.