Bronchoscopy for diagnosis of COVID-19 with respiratory failure: A case report

Qing-Yun Chen,Yu-Sheng He,Kai Liu,Jing Cao,Yong-Xing Chen

Qing-Yun Chen,Kai Liu,Yong-Xing Chen,Department of Respiratory and Critical Care Medicine,Hainan General Hospital,Haikou 570311,Hainan Province,China

Yu-Sheng He,Department of Internal Medicine,Hainan Province's Fucheng Drug Rehabilitation Centre,Haikou 570311,Hainan Province,China

Jing Cao,Infection Department,Hainan General Hospital,Haikou 570311,Hainan Province,China

Abstract BACKGROUND Although the imaging features of coronavirus disease 2019 (COVID-19) are starting to be well determined,what actually occurs within the bronchi is poorly known.Here,we report the processes and findings of bronchoscopy in a patient with COVID-19 accompanied by respiratory failure.CASE SUMMARY A 65-year-old male patient was admitted to the Hainan General Hospital on February 3,2020 for fever and shortness of breath for 13 d that worsened for the last 2 d.The severe acute respiratory syndrome coronavirus 2 nucleic acid test was positive.Routine blood examination on February 28 showed a white blood cell count of 11.02 × 109/L,86.9% of neutrophils,6.4% of lymphocytes,absolute lymphocyte count of 0.71 × 109/L,procalcitonin of 2.260 ng/mL,and C-reactive protein of 142.61 mg/L.Oxygen saturation was 46% at baseline and turned to 94%after ventilation.The patient underwent video bronchoscopy.The tracheal cartilage ring was clear,and no deformity was found in the lumen.The trachea and bilateral bronchi were patent,while the mucosa was with slight hyperemia;no neoplasm or ulcer was found.Moderate amounts of white gelatinous secretions were found in the dorsal segment of the left inferior lobe,and the bronchial lumen was patent after sputum aspiration.The right inferior lobe was found with hyperemia and mucosal erosion,with white gelatinous secretion attachment.The patient’s condition did not improve after the application of therapeutic bronchoscopy.CONCLUSION For patients with COVID-19 and respiratory failure,bronchoscopy can be performed under mechanical ventilation to clarify the airway conditions.Protection should be worn during the process.Considering the risk of infection,it is not necessary to perform bronchoscopy in the mild to moderate COVID-19 patients.

Key Words: COVID-19; Respiratory failure; Bronchoscopy; Diagnosis; Treatment; Case report

INTRODUCTION

Coronavirus disease 2019 (COVID-19) is a global pandemic acute respiratory disease that first appeared in Wuhan (Hubei,China) in December 2019[1,2].The pandemic spread rapidly to nearly all countries around the world,with 3917366 confirmed cases and 274361 deaths as of May 10,2020[3].On January 7,2020,genomic sequencing of the pathogens isolated from the airway samples of patients was firstly conducted by Chinese investigators,and the findings demonstrated that the epidemic was caused by a novel coronavirus[4].The virus was named severe acute respiratory syndrome coronavirus 2 by the International Committee on Taxonomy of Viruses[5].

The disease is mainly transmitted by respiratory droplets and close contacts and is transmitted from humans to humans[6].The common signs of COVID-19 include fever,cough,and shortness of breath[2].The severity of the COVID-19 could be classified as mild,regular,severe,and critical[7].There is no specific antiviral treatment for COVID-19,but supportive care may help relieve symptoms and should include support of vital organ functions in severe cases[2].Severe and critical cases require oxygen support and mechanical ventilation[2].Death is usually due to respiratory failure,but emerging evidence indicates that inflammation,thromboembolism,and multi-organ failure could be involved[1,2,8].

Although the imaging features of COVID-19 are starting to be well determined[9-11],what actually occurs within the bronchi is poorly known.Bronchoscopy adds important information in this regard but carries a high risk of aerosol spread of the virus,so its use must be carefully evaluated before use in emergency and necessary situations such as unexplained increase in airway pressure or lung collapse[12,13].Here,we report the processes and findings of bronchoscopy in one patient with COVID-19 accompanied by respiratory failure.In addition,the related literature is reviewed to provide evidence in helping understand the advantages and disadvantages of bronchoscopy in such cases,as well as improving the bronchoscopy in COVID-19 patients.

CASE PRESENTATION

Chief complaints

A 65-year-old male patient was admitted to the Hainan General Hospital on February 3,2020 for fever and shortness of breath for 13 d that worsened for the last 2 d.The patient is from Wuhan,Hubei,traveled to Haikou by plane on January 21,2020,and then drove to Danzhou,Hainan using a rented car.

History of present illness

The fever appeared 13 d before the admission,with the highest body temperature being 38.0 °C,which was accompanied by chilling,shortness of breath,headache,and weakness.No cough,expectoration,chest distress,or chest pain was reported.The severe acute respiratory syndrome coronavirus 2 nucleic acid test was initially performed at the Danzhou People’s Hospital and was positive.Due to the shortness of breath worsening,the patient was transferred to the Hainan General Hospital.

History of past illness

The patient had a 20-year history of hypertension and was treated with oral metoprolol 23.75 mg/d.The patient underwent minimally invasive surgery for atrial fibrillation 2 years before at Wuhan Union Hospital.

Personal and family history

The patient was born and raised in Wuhan without family history of genetic disease,psychosis,and tumor.

Physical examination

Physical examination showed that the body temperature was 36.0 °C,pulse was 110 beats/min,respiration was 30 breaths/min,blood pressure was 179/99 mmHg,and blood oxygen saturation was 46%.

Laboratory examinations

Tracheal intubation was conducted immediately after hospitalization,and mechanical ventilation was used.The mechanical ventilator settings were assisted ventilation,synchronized intermittent mandatory ventilation (SIMV) mode,FiO250%,ventricular tachycardia (VT) 480 mL,respiratory rate (f) 20 times/min,polystyrene (PS) 12 cmH2O,and positive end expiratory pressure (PEEP) 5 cmH2O.Blood gas analysis after inserting the ventilator showed that the partial pressure of carbon dioxide (PCO2)was 44.00 mmHg and partial pressure of oxygen was 89.00 mmHg.After 20 d of oral tracheal intubation,a tracheotomy was performed,and mechanical ventilation was continued.Blood routine examination on February 28,2020 showed that the white blood cell count was 11.02 × 109/L,the neutrophil percentage was 86.9%,lymphocyte percentage was 6.4%,absolute lymphocyte count was 0.71 × 109/L,fungal glucan (Gtest) was 231.5 pg/mL,procalcitonin was 2.260 ng/mL,and C-reactive protein was 142.61 mg/L.Sputum smear examination showed a moderate amount of Gramnegative bacilli and a small amount of Candida,indicating ventilator-associated pneumonia in addition to viral pneumonia.The blood gas analysis showed that the pH value was 7.295,PCO2was 60.70 mmHg,the partial pressure of oxygen was 81.00 mmHg,actual bicarbonate was 29.50 mmol/L,and oxygen saturation was 94.00%.

Imaging examinations

Chest imaging was completed (Figure 1).For patients with pulmonary exudation,bronchoscopy can be used to observe the condition of airway secretions and to remove them.During the hospitalization,the patient changed from type I to type II respiratory failure,and there was insufficient ventilation.Therefore,bronchoscopy was carried out to determine the cause of airway problems and observe for lesions.The patient underwent video bronchoscopy [QG-3490 (4.8-2.2); Zhuhai seesheen Medical Technology Co.,Ltd.,Zhuhai,China].The one-piece protective suit and full-face respiratory protective device were worn according to the level-3 protection standards for infectious diseases before entering the negative-pressure ward.Intravenous injection of midazolam (3 mg) was performed at 5 min before bronchoscopy,and propofol (30 μg/min/kg) and remifentanil (0.1 μg/min/kg) were used for sedation and analgesia.The richmond agitation-sedation scale score of the patient was maintained at -4 points.The mechanical ventilator was set to the SIMV mode,with FiO2100%,VT 480 mL,f 20 times/min,PS 12 cmH2O,and PEEP 5 cmH2O.Sterilized paraffin oil was applied to the surface of the bronchoscope,which was inserted through the working access of the universal joint that was connected to the tracheal catheter.

Figure 1 Chest imaging.A: Bedside chest image of the patient,taken on February 3,2020,i.e.,the 13th day of the disease,showing multiple patchy ground-glass opacities in the bilateral lungs,and some of them were with consolidation; B: Chest image of the patient,taken on February 28,2020,i.e.,the 38th day of the disease,showing multiple exudative lesions in bilateral lungs and local consolidations,and the lesions in lateral strips of bilateral lungs increased.

FINAL DIAGNOSIS

The tracheal cartilage ring was clear,and no deformity was found in the lumen.The trachea and bilateral bronchi were patent,while the mucosa was with slight hyperemia; no neoplasm or ulcer was found.Moderate amounts of white gelatinous secretions were found in the dorsal segment of the left inferior lobe,and the bronchial lumen was patent after sputum aspiration.The right inferior lobe was found with hyperemia and mucosal erosion,with white gelatinous secretion attachment(Figure 2).

TREATMENT

The patient received intravenous injection of midazolam (3 mg) 5 min before video bronchoscopy [QG- 3490 (4.8-2.2); Zhuhai Seesheen Medical Technology Co.,Ltd.,Zhuhai,China] to determine the cause of airway problems and observe for lesions.Propofol (30 μg/min/kg) and remifentanil (0.1 μg/min/kg) were used for sedation and analgesia.The richmond agitation-sedation scale score of the patient was maintained at -4 points.The mechanical ventilator was set to the SIMV mode,with FiO2100%,VT 480 mL,f 20 times/min,PS 12 cmH2O,and PEEP 5 cmH2O.Sterilized paraffin oil was applied to the surface of the bronchoscope,which was inserted through the working access of the universal joint that was connected to the tracheal catheter.

OUTCOME AND FOLLOW-UP

The patient’s condition did not improve after the application of therapeutic bronchoscopy.

DISCUSSION

The condition of the patient deteriorated,prompting hospitalization,and he was in a critical condition because of respiratory failure on admission.This deterioration could be due to his advanced age,delayed hospitalization,and the existence of comorbidities such as hypertension.Of course,the natural history of COVID-19 is still poorly understood,and additional aggravation factors could have been missed.Angiotensinconverting enzyme inhibitors were not used by the patient,and the disease severity was not significantly associated with anti-hypertensive drugs such as angiotensinconverting enzyme inhibitors[14].

Figure 2 Bronchoscopic findings.A: The carina was smooth and sharp,and the mucosa was with slight hyperemia; B: The left principal bronchus was with slight hyperemia.The lumen was patent and without secretions; C: The mucosa of the bronchi of the left superior lobe was with slight hyperemia.The lumen was patent and without secretions; D: The mucosa of the dorsal bronchi of the left inferior lobe was with slight hyperemia and a small amount of white gelatinous secretion; E: The left principle bronchus was with slight hyperemia,and the lumen was patent; F: The bronchi of the right inferior lobe were with hyperemia and mucosa erosion,as well as the attachment of white gelatinous secretions.

During COVID-19,the pulmonary exudation in this patient was evident.During disease progression,the blood oxygen saturation decreased to dramatic levels,and PCO2increased,indicated poor ventilation.The patient was diagnosed with critical COVID-19 accompanied with type II respiratory failure that required mechanical ventilation.For pneumonia patients with ventilation and gas-exchange dysfunctions,bronchoscopy might help clarify the airway conditions,identify the hidden pulmonary lesions,and remove airway secretions,which could improve ventilation to some extent[15].Bronchoscopy in this patient showed no secretions in the trachea and bilateral bronchi,while the mucosa was with slight hyperemia.Moderate amounts of white gelatinous secretions were found in the dorsal segment of the left inferior lobe.These findings were generally in agreement with the autopsy findings of COVID-19 patients[16].The findings also suggested that the airway secretions were from the respiratory bronchiole,namely,the small terminal airways,alveolar ducts,alveolar sacs,and alveoli,which have ventilation and gas-exchange functions.These structures have no goblet cells with high mucosa-secretion functions and are where COVID-19 seems to occur[17].These findings could explain the low blood oxygen saturation and increased PCO2in the patient.

The patient was with ventilator-associated pneumonia in addition to viral pneumonia.Tracheal intubation and tracheotomy provide conditions favoring the bacteria breeding,damages of the respiratory mucosal barrier,and reduction of immunity.Ventilator-associated pneumonia is the most common complication of mechanical ventilation[18].After the occurrence of ventilator-associated pneumonia,diagnostic bronchoscopy could shorten antibiotic treatment and hospital stay[19].Nevertheless,in this case,the application of therapeutic bronchoscopy achieved no evident benefits.

Nevertheless,the bronchoscopy clarified the airway conditions of the patients with critical COVID-19 accompanied by type II respiratory failure.In addition,bronchoscopy could directly visualize the lesion and accurately and completely remove the airway secretions,but the damage was relatively mild.Therefore,bronchoscopy could avoid the aimlessness of routine sputum suction and increase ventilation efficiency[20].A bronchoscopic examination might be necessary for critical COVID-19 patients accompanied by respiratory failure.In order to ensure the safety of patients,the ventilator should not be stopped during the procedures,and the oxygen concentration should be increased at the same time to ensure the oxygen supply during the procedures.Nevertheless,it has to be noted that some societies issued guidelines about the use of bronchoscopy in the context of COVID-19[12,21-23],but none address specifically the case of diagnostic and therapeutic bronchoscopy in patients with COVID,mainly because of the lack of data[24].

It is important to note that bronchoscopy is not recommended in patients with mild COVID-19[12,13].COVID-19 is transmitted through respiratory droplets and close contacts[7].The bronchoscopy physician could come in contact with the respiratory secretions,and bronchoscopy might induce aerosol spread,and thus the risk is extremely high.It has been suggested that bronchoscopy in patients with COVID-19 should be strictly kept for emergency and necessary situations[12,13].In this case,bronchoscopy was performed because the patient’s pCO2was still elevated under invasive mechanical ventilation,that is,type II respiratory failure.Because this patient has already undergone invasive mechanical ventilation,bronchoscopy was easier to perform.Therefore,in similar cases protection is very important,not only for the physicians and personnel but also to prevent the spread of the virus to unaffected patients[25].The following items could be considered.First,level-3 protection standards for infectious diseases should be used by the operators,and a full-face respiratory protective device should be worn.Second,before the examination,the patients should receive sedatives and analgesics,which could ease the nervousness,reduce cough,and decrease the spraying of droplets,thus protecting the operators and increasing the safety and comfort of the procedures.Third,the bronchoscope was inserted through the working access of the universal joint connected to the tracheal catheter,and paraffin oil was applied to the surface of the bronchoscope,which could increase the smoothness of the procedures and also help isolate the airway and external environment.Finally,the skillfulness of the operators and a gentle and swift operation could shorten the time of procedures in the trachea as possible.

CONCLUSION

For mild to moderate COVID-19 patients,there is no need for bronchoscopy,while for patients with respiratory failure and high amounts of exudative lesions in the lung,bronchoscopy examinations can be necessary under respiratory assistance by the ventilator.Nevertheless,the operators are at high risk of infection when performing bronchoscopic diagnosis and treatment for COVID-19 patients.In addition,the airway secretions in such patients might be limited.Therefore,frequent examinations in such patients are not necessary,and bronchial lavage could be performed according to the amount of airway secretions.