不同生物藥劑對茶炭疽病菌和茶輪斑病菌的室內毒力測定

郭遲鳴 林文珍 汪文華 郭鶯 劉黎卿 林志楷

摘要 ? ?茶樹是我國重要的經濟作物，但是由于其主要生長在溫暖、濕潤的環境，造成了茶樹病害的滋生。茶炭疽病和茶輪斑病是茶樹的重要病害，目前主要利用化學方法防治，而生物防治方法仍十分有限。本研究利用菌絲生長速率法測定了4種生物藥劑對茶炭疽病菌和茶輪斑病菌的室內抑菌活性。結果表明，0.5%香菇多糖對茶炭疽病菌和茶輪斑病菌菌絲生長的抑制作用較好，EC50值分別為9.40 μg/mL和2.68 μg/mL;其次為6%阿泰靈，EC50值分別為18.25 μg/mL和18.63 μg/mL，而5%海島素和3%植物免疫蛋白效果較差。

關鍵詞 ? ?茶炭疽病菌;茶輪斑病菌;生物藥劑;毒力測定

中圖分類號 ? ?S435.711 ? ? ? ?文獻標識碼 ? ?A

文章編號 ? 1007-5739（2020）12-0116-02 ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? 開放科學（資源服務）標識碼（OSID）

Toxicity ?Measurement ?of ?Different ?Biological ?Reagents ?on ?Colletotrichum ?gloeosporioides ?and ?Pseudopestalotiopsis

camelliae-sinensis

GUO Chi-ming 1，2 ? ?LIN Wen-zhen 1，2 ? ?WANG Wen-hua 1，2 ? ?GUO Ying 1，2 ? LIU Li-qing 1，2 ? ?LIN Zhi-kai 1，2

（1 Fujian Institute of Subtropical Botany，Xiamen Fujian 361006; 2 Fujian Key Laboratory of Physiology and Biochemistry for Subtropical Plant）

Abstract ? ?Tea plant is one of the important economic plants in China， it mainly grows in warm and humid environment， which resulting in the disease propagation. Anthracnose and gray leaf blight leaf spot are two important diseases in tea plants.At present， controlling the two diseases mainly relies on chemical fungicides， but biological control methods are still very limited. The paper used the mycelium growth rate method to determine inhibitory effect of 4 different biological reagents against Colletotrichum gloeosporioides and Pseudopestalotiopsis camelliae-sinensis. The results showed that 0.5% lentinan had the strongest anitfungal activity on the mycelial growth of Colletotrichum gloeosporioides and Pseudopestalotiopsis camelliae-sinensis， and the EC50 values were 9.40 μg/mL and 2.68 μg/mL， respectively; 6% Atailing also showed good inhibitory activity on the two fungus， the EC50 values were 18.25 μg/mL and 18.63 μg/mL， respectively. While 5% Haidaosu and 3% plant immune protein showed poor inhibitory activity.

Key words ? ?Colletotrichum gloeosporioides;Pseudopestalotiopsis camelliae-sinensis;biological reagent;toxicity measurement

茶樹作為一種重要的經濟作物，廣泛種植于熱帶和亞熱帶地區。我國是茶樹的原產地，茶葉更是我國對外貿易的重要農產品之一[1]。茶葉品質及農殘是制約茶葉生產出口的重要因素，因而篩選和使用高效、低殘留的農藥對有效合理防治茶樹病害具有重要意義。