基于網絡藥理學的安腸愈瘍湯治療潰瘍性結腸炎作用機制研究

樊靜娜

摘要：目的 ?基于網絡藥理學探討安腸愈瘍湯治療潰瘍性結腸炎（ulcerative colitis，UC）的作用機制。方法 ?通過中醫藥整合藥理學研究平臺構建安腸愈瘍湯的潛在靶標與UC疾病靶標之間的相互作用網絡。基于GO數據庫和KEGG通路數據庫進行基因功能和通路富集，采用Cytoscape3.6.1軟件構建靶標-信號通路網絡、藥物活性成分-靶標網絡，采用熒光實時定量PCR對網絡藥理學主要分析結果進行驗證。結果 ?分析結果表明，安腸愈瘍湯靶點主要關聯UC炎癥因子及免疫調控，糖類、脂質和氨基酸代謝，能量代謝，神經-內分泌系統調節等;體外實驗提示，安腸愈瘍湯參與調控GCK、CROT介導的糖脂代謝，GNB1介導的炎癥通路，以及ATP1A1、COX7C介導的能量代謝、氧化磷酸化、線粒體電子呼吸鏈傳遞等通路。結論 ?安腸愈瘍湯治療UC具有多成分、多靶點的特點，可為進一步研究其作用機制提供依據。

關鍵詞：安腸愈瘍湯;潰瘍性結腸炎;網絡藥理學;作用機制

中圖分類號：R259.746.2;R2-05 ???文獻標識碼：A??? 文章編號：1005-5304（2020）01-0092-06

DOI：10.3969/j.issn.1005-5304.201902170

Study on Mechanism of AnchangYuyang Decoction in Treating Ulcerative ColitisBased on Network Pharmacology

FAN Jingna

First Clinical Medical School， Shandong University of Traditional Chinese Medicine， Jinan 250014， China

Abstract： Objective To explore the mechanism of Anchang Yuyang Decoction in the treatment of ulcerative colitis （UC） based on network pharmacology. Methods The interaction network between UC targets and potential targets of Anchang Yuyang Decoction was constructed through Integrative Pharmacology-based Research Platform of TCM. Gene function and pathway enrichment were conducted based on GO database and KEGG pathway database. Cytoscape3.6.1 was used to construct target-signal pathway network and pharmaceutically active component-target network. The main results of network pharmacology were verified by real-time quantitative PCR. Results The results showed that the targets of Anchang Yuyang Decoction were mainly associated with inflammatory chemokines and immune system regulation in UC， carbohydrates， lipids and amino acid metabolism， energy metabolism， neuro-endocrine system regulation， etc. Vitro experiments indicated that Anchang Yuyang Decoction was involved in the regulation of glucose and lipid metabolism mediated by GCK and CROT， inflammatory pathway mediated by GNB1， as well as energy metabolism， oxidative phosphorylation， mitochondrial electron respiratory chain transmission mediated by ATP1A1 and COX7C. Conclusion AnchangYuyang Decoction applied for treating UC has the advantages of multi-components and targets， which can provide references for further research on mechanism.

Keywords： Anchang Yuyang Decoction; ulcerative colitis; network pharmacology; mechanism

潰瘍性結腸炎（ulcerative colitis，UC）是一種以結腸和直腸黏膜彌漫性炎癥為特征的慢性非特異性炎癥性腸病。本病臨床癥狀以發作、緩解交替出現為特點，易復發，且部分重癥患者病情重、變化快，藥物治療效果不佳。目前，現代醫學對UC病因及發病機制未完全闡明，尚無針對其病因的特效藥物。中醫通過辨病與辨證相結合，抓住UC病機特點，隨證加減用藥，可顯著改善UC患者生活質量，維持疾病臨床緩解，防治并發癥，并降低疾病復發率^[1]。

安腸愈瘍湯是導師遲莉麗教授經多年臨床實踐總結的經驗方，藥用黃芪、薏苡仁、白術、黃芩、黃連、木香、檳榔、地榆、當歸、防風、白芍、白及、甘草，共奏扶正補虛、解毒安腸之功。……