電針介入時機對腰多裂肌損傷模型大鼠Foxo1、Myostatin、Myod蛋白表達的影響

張佳怡　王彤　白玉琢　李欣怡　魯曼　陳潔　許玥　張淑靜　張莉　劉通

摘要?目的：觀察不同電針介入時機對腰多裂肌損傷大鼠多裂肌叉頭蛋白（Foxo1）、肌肉生長抑制素（Myostatin）、成肌分化因子（Myod）蛋白表達的影響。方法：雄性SD大鼠隨機分為空白組、模型組、即刻電針組、24 h電針組、48 h電針組，每組8只。以多裂肌肌注0.5%布比卡因復制腰多裂肌損傷模型。各電針組分別在造模后即刻、24 h、48 h開始電針雙側“委中”“腎俞”，連續干預7 d。以Western blot檢測各組多裂肌Foxo1、Myostatin、Myod蛋白表達水平。結果：與空白組比較，模型組Foxo1、Myod蛋白表達水平顯著升高（P<0.01）;與模型組比較，即刻電針組、48 h電針組Foxo1、Myostatin蛋白表達水平降低（P<0.01，P<0.05），24 h電針組Foxo1、Myostatin蛋白表達水平顯著降低（P<0.01），Myod蛋白表達水平顯著升高（P<0.01）;與24 h電針組比較，即刻電針組Myostatin蛋白表達水平升高（P<0.05）、Myod蛋白表達水平顯著降低（P<0.01），48 h電針組Foxo1蛋白表達水平顯著升高（P<0.01）、Myod蛋白表達水平顯著降低（P<0.01）。結論：電針促進多裂肌修復的最佳介入時機可能為損傷后24 h。

關鍵詞?電針;針刺時機;多裂肌損傷;叉頭蛋白;肌肉生長抑制素;成肌分化因子;肌衛星細胞;骨骼肌修復

Effects of Electroacupuncture Intervention Time on the Expression of Foxo1，Myostatin and Myod in Rats with Multifidus Muscle Injury

Zhang Jiayi1，Wang Tong1，Bai Yuzhuo1，Li Xinyi1，Lu Man1，Chen Jie1，Xu Yue1，Zhang Shujing2，Zhang Li1，Liu Tong3

（1 College of Acupuncture-Moxibustion and Tuina，Beijing University of Chinese Medicine，Beijing 100029，China; 2 School of Preclinical Medicine，Beijing University of Chinese Medicine，Beijing 100029，China; 3 The Fifth Clinical Medical College of Guangzhou University of Chinese Medicine，Guangzhou 510000，China）

Abstract?Objective：To observe the effects of different electroacupuncture intervention time on the expression of multifidus muscle fork protein（Foxo1），Myostatin and myoblast differentiation factor（Myod）in rats with lumbar multifidus muscle injury. Methods：Male Sprague-Dawley rats were randomly divided into the blank group（BG），model group（MG），immediate/24 h/48 h electroacupuncture group（0 hG/24 hG/48 hG），with 8 rats in each group. The lumbar multifidus muscle injury model was replicated with 0.5% bupivacaine in the multifidus muscle. Each electroacupuncture group started the electroacupuncture on both sides of the Weizhong（BL40）and Shenshu（BL23）at 0 h/24 h/48 h after modeling，and continuous intervention for 7 days. Western blot was used to detect the expression levels of Foxo1，Myostatin and Myod proteins in multifidus muscles of each group. Results：Compared with the BG，the expression of Foxo1 and Myod proteins in the MG significantly increased（P<0.01）; Compared with the MG，the expression of Foxo1 and Myostatin in the 0 hG and 48 hG were decreased（P<0.01，P<0.05）. The expression of Foxo1 and Myostatin in the 24 hG significantly decreased（P<0.01），and Myod protein expression was significantly increased（P<0.01）. Compared with the 24 hG，the expression of Myostatin protein in 0 hG increased（P<0.05），and the expression of Myod protein in 0 hG significantly decreased（P<0.01）; the expression of Foxo1 protein in 48 hG was significantly increased（P<0.01），and the expression of Myod protein was significantly decreased（P<0.01）. Conclusion：The best intervention time is 24 h after injury，wich can effectively promote multifidus muscle repairing.

Key Words?Electroacupuncture; Acupuncture time; Multifidus muscle injury; Fork protein; Myostatin; Myogenic differentiation factor; Muscle satellite cell; Skeletal muscle repair

中圖分類號：R274.3;R245.3文獻標識碼：Adoi：10.3969/j.issn.1673-7202.2019.03.016

多裂肌是附著面積最大的椎旁肌，對維持腰椎穩定以及控制脊柱活動起到關鍵性的作用。近年來越來越多國內外學者聚焦于多裂肌的康復和功能訓練[1-2]，以期通過促進多裂肌損傷后修復改善腰背功能障礙。骨骼肌損傷后修復主要依賴骨骼肌干細胞-肌衛星細胞（Muscle Satellite Cells，MSCs）的增殖與分化。研究證明[3-4]MSCs的增殖與分化過程有多種細胞因子及蛋白的參與，其中叉頭蛋白（Foxo1）、肌肉生長抑制素（Myostatin）及成肌分化因子（Myod）均與MSCs的增殖密切相關。……