隔藥餅灸對(duì)慢性支氣管炎模型大鼠肺組織內(nèi)p-p38MAPK、ICAM-1的影響

魏星　譚靜　言芳　孫希　孫璐　羅明　陽(yáng)仁達(dá)

摘要?目的：觀察隔藥餅灸對(duì)慢性支氣管炎大鼠p-p38MAPK、ICAM-1的影響。方法：將48只SD大鼠隨機(jī)分成空白組、模型組、隔藥餅灸組、隔紙墊灸組，每組12只。選用改良煙熏法建立大鼠慢性支氣管炎模型，干預(yù)后，測(cè)定肺泡灌洗液中性粒細(xì)胞含量，各組大鼠ICAM-1 mRNA選用RT-PCR術(shù)測(cè)定、ICAM-1及p-p38MAPK蛋白選用Western blot檢測(cè)。結(jié)果：與空白組比較，模型組中性粒細(xì)胞、p-p38MAPK、ICAM-1 mRNA及蛋白含量增高顯著（P<0.01）。與模型組比較，隔藥餅灸組中性粒細(xì)胞數(shù)目減少（P<0.05），p-p38MAPK、ICAM-1 mRNA及蛋白含量顯著降低（P<0.01）。與隔紙墊灸組比較，隔藥餅灸組中性粒細(xì)胞、p-p38MAPK蛋白、ICAM-1 mRNA及蛋白降低更明顯（P<0.05）。結(jié)論：隔藥餅灸可通過(guò)減少p-p38MAPK蛋白含量、降低ICAM-1 mRNA及其蛋白水平，抑制中性粒細(xì)胞的釋放，以改善炎性反應(yīng)環(huán)境治療慢性支氣管炎。

關(guān)鍵詞?隔藥餅灸;慢性支氣管炎;中性粒細(xì)胞;ICAM-1;p-p38MAPK;大鼠;模型

Effects of Cake-separated Moxibustion on p-p38MAPK and ICAM-1 in Rats′ Lung Tissue with Chronic Bronchitis

Wei Xing，Tan Jing，Yan Fang，Sun Xi，Sun Lu，Luo Ming，Yang Renda

（Hunan University of Chinese Medicine，Changsha 410208，China）

Abstract?Objective：To observe the effects of cake-separated moxibustion on intercellular adhesion molecule-1（ICAM-1）and p-p38MAPK in rats with chronic bronchitis. Methods：A total of 48 Sprague-Dawley（SD）rats were randomly separated into the blank group，model group，cake-separated moxibustion group and paper pad-separated moxibustion team（12 in each group）. In addition to the blank group，other 3 groups used modified fumigation to establish the chronic bronchitis models of rats. After intervention，the content of neutrophils in Bronchoalveolar Lavage Fluid（BALF）was measured. ICAM-1 mRNA was detected by RT-PCR，while protein contents of ICAM-1 and p-p38MAPK were detected by Western blot. Results：Compared with the blank group，the contents of neutrophils，p-p38MAPK and ICAM-1 mRNA were obviously increased in the model group（P<0.01）. Compared with the model group，the number of neutrophils was decreased（P<0.05）and the contents of p-p38MAPK，ICAM-1 mRNA were significantly decreased（P<0.01）. Compared with the paper pad-separated moxibustion group，the contents of neutrophils，p-p38MAPK，ICAM-1 mRNA and protein were significantly decreased in the cake-separated moxibustion group（P<0.05）. Conclusion：Cake-separated moxibustion can inhibit the release of neutrophils and significantly reduce the inflammatory reaction，by reducing the contents of p-p38MAPK，ICAM-1 mRNA and protein，so as to treat chronic bronchitis.

Key Words?Cake-separated moxibustion; Chronic bronchitis; Neutrophil; ICAM-1; p-p38MAPK; Rat; Model

中圖分類號(hào)：R245.8文獻(xiàn)標(biāo)識(shí)碼：Adoi：10.3969/j.issn.1673-7202.2019.03.009

慢性支氣管炎（Chronic Bronchitis，CB）是以反復(fù)發(fā)作的咳嗽、咯痰、常伴喘息為主要特點(diǎn)的非特異性炎性病癥。近年來(lái)，隨著環(huán)境的變化和不良的生活習(xí)慣，CB的發(fā)病率在全球范圍內(nèi)持續(xù)走高，且易進(jìn)展成肺氣腫、肺心病等疾病，對(duì)患者生存生活造成威脅[1-2]。盡管CB的病因復(fù)雜，但其病理改變十分明確，即以氣道的炎性反應(yīng)為典型，病變部位常聚集有大量中性粒細(xì)胞。這些細(xì)胞通過(guò)作用于支氣管內(nèi)皮細(xì)胞，促進(jìn)其釋放ICAM-1、蛋白酶等物質(zhì)，這個(gè)過(guò)程主要受p38MAPK通路調(diào)節(jié)，激活后能釋放系列炎性因子，大量炎性反應(yīng)因子又能反作用進(jìn)一步激活上述通路，導(dǎo)致炎性反應(yīng)瀑布反應(yīng)[3]。……