艾灸對胃荷瘤大鼠外周血中CD4+、CD4+CD25+T淋巴細胞的影響

譚靜　林亞平　趙歡　彭卓雋　歐陽里知　陳欲攀　石俊林

摘要?目的：觀察艾灸對胃荷瘤大鼠外周血中CD4+、CD4+CD25+T淋巴細胞亞群的影響。方法：將50只健康SPF級SD大鼠，雌雄各半，隨機分為空白組、假手術組、模型組、艾灸組和紅外組5組，每組10只。利用Walker-256細胞來源的實體瘤組織移植胃部制備模型。模型成功后，艾灸組予以懸灸中脘、胃俞等穴位;紅外組予以短波紅外線照射腹部胃脘及背部胃脘投影區;其余組捆綁束縛。干預20 min/次，1次/d，持續21 d。干預結束后，眼眶采血，流式細胞技術測定外周血中CD4+、CD4+CD25+T淋巴比例。處死動物，測算胸腺指數及腫瘤體積生長抑制率。結果：與空白組比較，模型組動物生存狀態變差，胸腺指數增加，外周血中CD4+CD25+T淋巴細胞比例顯著增加（P<0.05）;與模型組比較，艾灸組動物艾灸組動物生存狀態改善，胸腺指數進一步增加，瘤體內壞死灶較多，血管較少，外周血中CD4+T淋巴細胞比例顯著增加（P<0.01），CD4+CD25+T淋巴細胞比例減少（P<0.01）;與紅外組比較，艾灸組瘤體內壞死灶更多，血管較少，外周血中CD4+T淋巴細胞比例增加（P<0.01）。結論：艾灸可一定程度改善荷瘤動物生存狀態，減少腫瘤細胞轉移、促進腫瘤細胞壞死，減少血管增生。這種影響可能與增加CD4+T淋巴細胞，減少CD4+CD25+T淋巴細胞有關。

關鍵詞?艾灸;胃癌;胸腺指數;CD4+;CD4+CD25+;大鼠

Effects of Moxibustion on CD4+ and CD4+CD25+ T Lymphocytes in Peripheral Blood of Rats with Gastric Tumor

Tan Jing，Lin Yaping，Zhao Huan，Peng Zhuojun，Ouyang Lizhi，Chen Yupan，Shi Junlin

（School of Acupuncture，Moxibustion and Tuina，Hunan University of Chinese Medicine，Changsha 410208，China）

Abstract?Objective：To observe the effects of moxibustion on CD4+ and CD4+CD25+ T lymphocytes in peripheral blood of rats with gastric cancer. Methods：A total of 50 healthy SPF SD rats（half male and half female）were randomly divided into 5 groups：the blank group，the sham operation group，the model group，the moxibustion group and the infrared group，with 10 rats in each group. Solid tumor tissue derived from Walker-256 cells was transplanted into the stomach to prepare the tumor model. After modeling，moxibustion group and infrared group were treated 20 mins per day for 21 days. After the intervention，the blood from the orbits was collected. And the ratio of CD4+ and CD4+CD25+ T lymphocytes in peripheral blood was tested by flow cytometry. After killing the rats，the thymus index and the growth inhibition rate of the tumor volume were measured. Results：Compared with the blank group，the survival status of the model group was worse，the thymus index increased，and the proportion of CD4+CD25+ T lymphocytes in peripheral blood significantly increased（P<0.05）. Compared with the model group，the survival status of the moxibustion group was improved，the thymus index was further increased，more necrosis and less blood vessels were found in the tumors，and the percentage of CD4+ T lymphocytes was increased significantly（P<0.01），while that of CD4+CD25+ T lymphocyte was decreased（P<0.01）. Compared with the infrared group，more necrosis and few blood vessels were found in the tumors，and the proportion of CD4+ T lymphocytes was increased in the moxibustion group（P<0.01）. Conclusion：Moxibustion can improve the survival status of the rat with gastric tumor，inhibit tumor metastasis，promote tumor necrosis as well as prevent vessels hyperplasia. These changes may be related to the increasing CD4+ T lymphocytes and the decreasing CD4+CD25+ T lymphocytes.

Key Words?Moxibustion; Gastric cancer; Thymus index; CD4+; CD4+CD25+; Rat

中圖分類號：R245.8文獻標識碼：Adoi：10.3969/j.issn.1673-7202.2019.03.001

胃癌（Gastric Carcinoma）是消化道最常見的惡性腫瘤，常以上腹部不適、疼痛、嘔吐等為主要癥狀[1]。受地域環境及飲食習慣的影響，我國胃癌的發病率、死亡率遠高于全球平均水平[2]。胃癌的防治一直是我國醫學研究的重點。……