不同促透劑運(yùn)用于隔藥餅灸對(duì)高脂血癥兔血脂及炎性反應(yīng)因子的調(diào)節(jié)作用

廖宗力 孫璐 黃文韜

摘要 目的：氮酮和冰片作為促透劑運(yùn)用于藥餅中對(duì)高脂血癥兔進(jìn)行隔藥餅施灸，觀察其對(duì)高脂血癥兔血脂及炎性反應(yīng)因子的調(diào)節(jié)作用。方法：將40只新西蘭兔隨機(jī)分為正常組、高脂模型組、無(wú)促透劑組、氮酮組和冰片組，每組8只。正常組普通飼料喂養(yǎng)，其余各組用高脂飼料喂養(yǎng)12周成高脂模型，成模后采用不同促透劑運(yùn)用于隔藥餅灸施灸4周后，檢測(cè)兔血清總膽固醇（TC）、三酰甘油（TG）、高密度脂蛋白膽固醇（HDL-C）、低密度脂蛋白膽固醇（LDL-C）及TNF-α、INF-γ的含量。結(jié)果：3個(gè)隔藥餅灸組均顯著降低高脂模型兔血清TC、TG、LDL-C的含量（P<0.05）、顯著升高HDL-C的含量（P<0.05）;氮酮組降低TC和TG、冰片組降低TC、TG、LDL-C的效果優(yōu)于無(wú)促透劑組（P<0.05）;冰片組降低TC、TG、LDL-C的效果優(yōu)于氮酮組（P<0.05）;3個(gè)隔藥餅灸組均能顯著降低高脂血癥兔TNF-α及INF-γ的水平（P<0.05），且氮酮組和冰片組降低TNF-α、INF-γ的療效優(yōu)于無(wú)促透劑組（P<0.05），冰片組降低TNF-α的效果優(yōu)于氮酮組（P<0.05）。結(jié)論：隔藥餅灸能有效調(diào)節(jié)高脂模型兔血脂及炎性反應(yīng)因子水平，氮酮和冰片作為促透劑運(yùn)用于藥餅后施灸對(duì)實(shí)驗(yàn)性高脂模型兔血脂及炎性反應(yīng)因子的調(diào)節(jié)作用更顯著，且冰片組作用比氮酮組更優(yōu)，值得進(jìn)一步研究。

關(guān)鍵詞 高脂血癥;隔藥餅灸;透皮吸收促進(jìn)劑;經(jīng)皮給藥系統(tǒng);炎性反應(yīng)因子;氮酮;冰片

Abstract Objective：To study the regulatory effect of penetration enhancers Azone and borneol applied with herbal cake-partitioned moxibustion on blood lipid and inflammatory factor levels，and to observe the regulatory effects on the levels of blood lipids and inflammatory factors of a hyperlipidemia rabbit model.Methods：A total of 40 New Zealand white rabbits were randomly divided into a normal group，a hyperlipidemia group，a moxibustion treated only hyperlipidemia group，an Azone plus moxibustion treated hyperlipidemia group and a borneol plus moxibustion treated hyperlipidemia group，with 8 rabbits each.The normal group rabbits were feed normally，whereas other rabbits were firstly treated with high-fat food for 12 weeks to generate hyperlipidemia model，followed by treatment of herbal cake-partitioned moxibustion applied with penetration enhancers for 4 weeks.Afterwards，rabbit serum was collected and measured for levels of total cholesterol（TC），tryglyceride（TG），high-density lipoprotein cholesterol（HDL-C），low-density lipoprotein cholesterol（LDL-C），TNF-α，and INF-γ.Results：All the 3 herbal cake-partitioned moxibustion groups significantly reduced blood TC，TG and LDL-C（P<0.05），and significantly increased HDL-L（P<0.05）.The effect of the Azone group decreasing TC and TG，and the borneol group decreasing blood TC，TG and LDL-C levels was better than no penetration enhancers group（P<0.05）.The borneol group was better than the Azone group in reducing TC（P<0.05）.The 3 herbal cake-partitioned moxibustion groups significantly reduced blood TNF-α，and INF-γ levels（P<0.05），and the Azone group and the borneol group were better than no penetration enhancers group（P<0.05）.The borneol group was better than the Azone group in reducing TNF-α（P<0.05）.Conclusion：Herbal cake-partitioned moxibustion can effectively regulate the blood lipid and inflammatory factors in the high-fat model rabbits.The application of Azone and borneol，as a penetration enhancer，have more significant regulatory effect on the levels of blood lipids and inflammatory factors of a hyperlipidemia rabbit model after herbal cake-partitioned moxibustion，and the Azone group was better than the borneol group，which deserves further study.565BAB24-E58C-4B62-BC45-301E26F8074C

Key Words Hyperlipidemia; Herbal cake-partitioned moxibustion; Penetration enhancer; Transdermal drug delivery; Inflammatory factor; Azone; Borneol

中圖分類(lèi)號(hào)：R245.81 文獻(xiàn)標(biāo)識(shí)碼：A doi：10.3969/j.issn.1673-7202.2019.10.013

高脂血癥（Hyperlipidemia，HLP）是由于機(jī)體內(nèi)脂質(zhì)代謝或轉(zhuǎn)運(yùn)異常所導(dǎo)致的血脂異常病癥，表現(xiàn)為血漿總膽固醇（TC）、三酰甘油（TG）、低密度脂蛋白膽固醇（LDL-C）一種或幾種升高，和（或）高密度脂蛋白膽固醇（HDL-C）降低。21世紀(jì)以來(lái)，社會(huì)經(jīng)濟(jì)飛速發(fā)展，伴隨著人們生活方式、飲食結(jié)構(gòu)的改變、生活節(jié)奏的加快及競(jìng)爭(zhēng)壓力的增長(zhǎng)，我國(guó)居民高脂血癥的發(fā)病率呈迅速增長(zhǎng)的趨勢(shì)[1].據(jù)調(diào)查顯示：我國(guó)高脂血癥總?cè)藬?shù)至2010年已超過(guò)1.6億，患病率約20%，其中低HDL-C患病率44.8%、高TG患病率為11.3%、高TC血癥為3.3%、高LDL-C血癥為2.1%[2]。

高脂血癥是專(zhuān)家公認(rèn)的心腦血管動(dòng)脈粥樣硬化重要的危險(xiǎn)因素[3]，尤其低密度脂蛋白膽固醇（LDL-C）在動(dòng)脈粥樣硬化形成中扮演關(guān)鍵的角色[4]。同時(shí)，高脂血癥還與脂肪肝、糖尿病、血栓等的發(fā)生有關(guān)聯(lián)。……