Fulminant liver failure following a marathon:Five case reports and review of literature

Wojciech Figiel,Marcin Morawski,Micha? Gr?t,Oskar Kornasiewicz,Grzegorz Niewiński,Joanna Raszeja-Wyszomirska,Maciej Krasnod?bski,Arkadiusz Kowalczyk,Wac?aw Ho?ówko,Waldemar Patkowski,Krzysztof Zieniewicz

Abstract

Key words: Heat Stroke; Hepatic insufficiency; Liver transplantation; Case report

INTRODUCTION

In recent years,marathons have gained widespread popularity and attract both,professional and homegrown runners.More than 800 races are held every year[1],usually during the spring and summer,when high external temperature and humidity can create unfavorable conditions for exercise.

Heat produced by the human body at rest is a by-product of baseline metabolism.Homeostatic mechanisms tend to maintain the core body temperature within a range of 37 ± 0.5 °C[2].The liver,the heart,and the brain are organs with high metabolic rates and the main heat generators in the body.This heat is spreadviathe circulation to other body parts.Additional heat generated by the muscles during exercise can be dissipated by radiation or conduction to prevent hyperthermic injury to organs only when the core temperature exceeds the external temperature.However,when the conditions are reversed,sweating and evaporation occur to achieve heat loss.Under unfavorable conditions (e.g.,high external temperature and humidity and/or strenuous exercise) sweating and evaporation may be inadequate with a consequent elevation in core body temperature and multi-organ failure,which is defined as exertional heat stroke (EHS).

Individuals with EHS show core temperature ＞ 40 °C with consequent loss of consciousness and multi-organ damage[3].Hepatic injury is common under such conditions; however,owing to the remarkable hepatic functional reserve,those affected might typically remain asymptomatic.However,acute liver failure has been reported in approximately 5% of patients with EHS[4].Orthotopic liver transplantation(OLTx) is indicated in these individuals[5],although a few reports have described spontaneous recovery in such patients[4].

CASE PRESENTATION

Between 2005 and 2017,4 patients (3 men and 1 woman) were referred to the Department of General,Transplant,and Liver Surgery at the Medical University of Warsaw,and 1 man was referred to the Department of General and Transplant Surgery in Szczecin following a marathon run with clinical and biochemical findings suggesting EHS-induced fulminant liver failure (FLF).

Case 1

A previously healthy 24-year-old man collapsed after a 10-km run and was admitted to a local hospital.On initial examination,he was conscious with body temperature of 39.9 °C and right upper quadrant abdominal tenderness,but no neurological symptoms.The patient reported slight upper abdominal discomfort lasting a few weeks before the marathon and also the use of protein and amino acid supplements as part of his preparations for the marathon.Blood tests performed on days 2 and 3 of hospitalization revealed sudden elevation of serum alanine aminotransferase (ALT)and aspartate aminotransferase (AST) (ALT from 430 U/L to 8166 U/L and AST from 1397 U/L to 7550 U/L) (Figure 1A),lactate dehydrogenase (LDH) activity elevation from 4349 U/L to 10096 U/L,creatine kinase (CK) of 135400 U/L (indicating rhabdomyolysis),as well as deterioration of the synthetic function of the liver represented by an increased international normalized ratio (INR) of 7.12 (Figure 1B).Rhabdomyolysis caused acute kidney injury and elevation of serum creatinine levels(2.08 mg/dL,4.70 mg/dL,and 6.28 mg/dL on days 1,2,and 3,respectively) and a reduced estimated glomerular filtration rate (from 41.7 mL/min/1.73 m2to 11.7 mL/min/1.73 m2).Owing to the progressive worsening of his clinical status and biochemical parameters,the patient was referred to the department where this study was performed on day 3.On admission to our department,the patient was completely conscious [Glasgow Coma Scale (GCS) = 15] and cooperative.He was anuric(serum creatinine = 5.29 mg/dL) with anasarca.His abdomen was soft with normal peristalsis with slight epigastric tenderness.Additionally,subconjunctival and subcutaneous right cubital fossa hemorrhage was observed.Triple-phase abdominal computed tomography (CT) showed mild pleural and pelvic effusions,and faint contrast enhancement of the liver parenchyma [without contrast:15 Hounsfield Units(HU),arterial phase:20 HU,portal phase:33 HU].

Case 2

A previously healthy 26-year-old woman collapsed after a marathon run at the seaside (ambient temperature of 25°C) and was admitted to a local hospital.On initial examination,she was unconscious,with body temperature of 42°C,and upper body muscle rigidity.She regained consciousness within a few hours after admission;however,she demonstrated psychomotor irritability and reported upper abdominal discomfort.Head CT was performed to evaluate her prolonged upper body muscle rigidity,and the study was unremarkable.Blood tests performed on day 3 revealed markedly elevated serum ALT and AST levels of 6161 U/L and 7322 U/L,respectively (Figure 1C),LDH of 38910 U/L,serum CK levels ＞ 40000 U/L (indicating rhabdomyolysis),as well as severe deterioration of the synthetic function of the liver represented by an increased INR of 18.1 on day 3 (Figure 1D).Owing to the severe hepatic impairment and rhabdomyolysis,the patient was referred to the department where this study was performed on day 3 to assess her suitability to undergo urgent liver transplantation.On admission to our department,the patient was stuporous.Her laboratory test results showed signs of acute renal failure (serum creatinine = 4.2 mg/dL) and disseminated intravascular coagulation (DIC,INR = 18.1,D-dimer =13310 μg/mL,28000 platelets/mm3,fibrinogen = 79 mg/dL) with no signs of hemorrhage or active bleeding.

Case 3

A previously healthy 27-year-old man collapsed after an 11-km marathon run and was admitted to a local hospital.On initial examination,he was unconscious,and his initial medical records did not show details of his temperature,neurological status,or the efforts made to reduce his body temperature.He regained consciousness within a few hours after admission and was asymptomatic except for upper abdominal discomfort.Owing to rapid elevation of liver function tests on day 2 (Figures 1E,1F),he was transferred to the department where this study was performed on day 2 for further evaluation.On admission to our department,the patient was fully conscious(GCS = 15) and only reported mild upper abdominal tenderness.His serum AST and ALT levels were high at 2916 U/L and 4085 U/L,respectively,LDH elevation to 3557 U/L,thrombocytopenia of 74000 platelets/mm3,and INR = 1.74.

Case 4

A previously healthy 33-year-old man collapsed after running several kilometers during a marathon (ambient temperature = 30 °C) and was admitted to a local hospital.On admission,he was comatose (GCS = 6) with blood pressure of 90/60 mmHg,as well as nuchal and upper body muscle rigidity.CT upon admission did not show any abnormalities of the central nervous system.His early medical records did not show any information regarding body temperature or endeavors to reduce hyperthermia.He regained consciousness within a few hours after admission and was treated conservatively with crystalloid infusion.Assessment performed on consecutive days showed laboratory tests indicating DIC (INR = 8.81,23000 platelets/mm3) accompanied by anterior chest wall hemorrhages without any signs of major bleeding.He was treated with fresh frozen plasma and intramuscular vitamin K1 injections.Blood tests performed on days 3 and 4 showed marked elevation of serum AST,ALT,and bilirubin levels (Figures 1G,1H).Owing to the development of anuria and deterioration of his condition,he was transferred to the intensive care unit where he was intubated and continuous veno-venous hemodialysis was initiated.He was transferred to the department where this study was performed on day 7 following the exertion.On admission to our department,he was unconscious (GCS =3) and was intubated.

Figure1 Laboratory findings following the exertion and liver transplantation.

Case 5

A previously healthy 39-year-old man fainted after running several kilometers during a marathon and was admitted to a local hospital.On admission,the patient was stuporous and presented with generalized seizures.However,clinical examination did not show any significant abnormalities,and body cooling efforts were not deemed necessary.His medical history revealed several-year treatment for bipolar disorder(carbamazepine,clomipramine,and piracetam).Owing to rapidly progressing liver failure (Figure 1I,1J) and rhabdomyolysis (CK = 15755 U/L,myoglobin ＞ 1000 μg/L),the patient was referred to the department where this study was performed on day 3 to assess his suitability to undergo liver transplantation.On admission to our department,he was conscious and cooperative but slightly drowsy.

FINAL DIAGNOSIS

In all five cases that were treated at our department,an exertion induced fulminant liver failure was confirmed the diagnostic follow up including other causes of fulminant liver failure.The diagnosis of heat stroke was established in accordance with criteria of Japanese Association of Acute Medicine Heat Stroke Committee Working Group[6].

TREATMENT

Case 1

Immediately after admission,the patient’s case was reviewed by the transplant team,and he was shortlisted for OLTx as an urgent recipient.Continuous renal replacement therapy was initiated to treat persistent rhabdomyolysis.The patient received cryoprecipitate and prothrombin complex concentrate transfusions,as well as parenteral vitamin K1 owing to severe coagulopathy (INR = 6.02) and thrombocytopenia (50000 platelets/mm3).OLTx was performed on day 4 following the exertion.The liver was procured from a deceased donor and a piggy-back technique with end-to-end biliary anastomosis was used.

Case 2

Body cooling was performed in the emergency department.The patient was examined by the transplant team and considered an appropriate candidate to receive urgent OLTx,which was performed (using the liver from a deceased donor) on day 5 following her initial admission to the previous hospital.A standard piggy-back technique and end-to-end biliary anastomosis were performed.

Case 3

The case was reviewed by the transplant team,and the patient was listed for urgent OLTx.

Case 4

Immediately after arrival to our department,his case was reviewed by our transplant team,and he was listed as an urgent candidate for OLTx.On day 9 following exertion,progression of liver failure necessitated the institution of the Prometheus liver support therapy system.

Case 5

The case was reviewed by the transplant team,and he was listed as a candidate for OLTx.The OLTx was performed on day 4 following the exertion.The liver was procured from a deceased donor,and a piggy-back technique with end-to-end biliary anastomosis was used.A burst abdomen necessitated a reoperation on days 11 and 22 after the OLTx.His perioperative period was complicated by renal insufficiency treated with continuous veno-venous hemofiltration on the 1stand 2ndpostoperative days.

OUTCOME AND FOLLOW-UP

Case 1

Liver and renal function recovered immediately after OLTx (day 5 onwards).Serum AST,ALT,bilirubin,and INR levels were normalized over a 30-d period following the OLTx (ALT = 63 U/L,AST = 31 U/L,bilirubin = 0.5 mg/dL,and INR = 0.98),and his serum creatinine level was sustained at 2.19 mg/dL.He was transferred from the surgical unit to the inpatient liver ward on post-transplantation day 10 and was discharged on postoperative day 30.He is presently 44 wk post-transplantation without any complaints.

Case 2

A rapid decrease in serum aminotransferases (ALT = 580 U/L and AST = 440 U/L on post-transplantation day 3 and restoration of hepatic synthetic function (INR = 1.37 on post-transplantation day 1) occurred following the OLTx.Persistent features of kidney failure (serum creatinine = 7.42 mg/dL on post-transplantation day 3) necessitated the initiation of hemodialysis over 2 d.Intra-abdominal hemorrhage necessitated 2 reoperations (on post-transplantation days 7 and 14).She was discharged on postoperative day 40 without any signs of liver or renal impairment.She is now 13 years post-transplantation.

Case 3

While awaiting a suitable graft,his biochemical parameters started improving,and his serum AST and ALT levels were 44 U/L and 584 U/L,respectively on day 9.He was discharged on day 9 following the exertion with no clinical and biochemical signs of liver or renal damage.

Case 4

On the following day after admission,the patient developed hypertensive urgency(blood pressure 320/220 mmHg) followed by a rapid decline in blood pressure requiring noradrenaline infusion.The incident was accompanied by bilaterally dilated pupils that soon became unreactive.CT showed intracerebral hemorrhage in the left parietal area.A second episode of severe elevation of blood pressure was observed,and the patient died on day 11 following the exertion.

Case 5

The patient was discharged on day 37 following OLTx after his laboratory test results showed normalization,with no signs of liver or renal function impairment.He is now 10 years post-transplantation.

DISCUSSION

This report discusses a case series comprising 5 previously healthy individuals who developed signs of EHS-induced FLF.All patients were screened for other causes of FLF including hepatitis B and C infection,Wilson’s disease,autoimmune hepatitis,and hemochromatosis.Owing to a lack of admission records in a few patients,it was not possible to determine whether all patients met Bouchama’s criteria for heat stroke[7],although all patients fulfilled the Japanese Association of Acute Medicine Heat Stroke Committee Working Group (JAAM-HS-WG) criteria[6]:The JAAM-HSWG definition includes patients exposed to high environmental temperature meeting at least 1 of the following criteria:(1) GCS of ≤ 14; (2) Serum creatinine or total bilirubin levels of ≥ 1.2 mg/dL; and (3) JAAM DIC score of ≥ 4[8].

All patients in this case series showed the classical evolution of the clinical course observed in such cases,comprising the following:(1) Hyperthermic phase(neurological disturbances associated with hyperthermia); (2) Hematological and enzymatic phase (leukocytosis and hypocoagulability); and (3) Renal and hepatic phase (acute liver and renal failure)[9].The mechanisms for heat-induced liver failure remain unclear; however,it has previously been suggested that heat-triggered systemic inflammation can cause multi-organ failure with consequent activation of multiple pathways promoting cell death including interleukin 1b and high mobility group box protein 1 downstream signaling[10,11].It is also known that the pathophysiology of EHS-induced liver failure is associated with ischemia-induced hypoxic hepatitis[12],which may be attributed to systemic hypoperfusion caused by shunting of blood to the skin,thrombosis of inflow vessels,or congestive heart failure[12].This hypothesis is supported in this study by the radiological finding of poor contrast enhancement in Case 1 suggesting flow disturbances in the liver parenchyma observed on day 3[4,5,12,16-32](Figure 1I) and significant hypotension (blood pressure 60/40 mmHg) upon admission in Case 4.

This study highlights 3 distinct clinical outcomes in these patients:(1) Death while awaiting a suitable graft; (2) OLTx; and (3) Spontaneous recovery with supportive treatment alone.These observations highlight the need for criteria to determine the necessity and urgency of OLTx.Currently,the King’s College Criteria (KCC) and the Clichy criteria are widely accepted prognostic indicators to select patients with FLF who require OLTx.Based on the KCC,OLTx should be performed in patients presenting with non-acetaminophen-induced acute liver injury when the prothrombin time is longer than 100 s (INR ＞ 6.5),or if any 3 of the following criteria are observed:(1) Prothrombin time ＞ 50 s (INR ＞ 3.5); (2) Age ＜ 10 years or ＞ 40 years; (3) Duration of jaundice before onset of encephalopathy ＞ 7 d; (4) Total serum bilirubin level ＞ 17.5 mg/dL; and (5) Etiology attributable to non-A and non-B hepatitis,halothane hepatitis,or idiosyncratic drug reactions[13].In this study,4 of 5 patients met the KCC within 3 d following exertion.Interestingly,the only patient who did not meet the KCC was Case 4 who recovered spontaneously.This observation suggests that KCC could potentially predict patient suitability for OLTx; however,further studies are warranted to verify this hypothesis.Patients with FLF are not routinely screened for Factor V levels at the hospital where this study was performed; therefore,identifying which patients met the Clichy criteria was not possible[14].Furthermore,the Clichy criteria were designed to predict outcomes in patients with acute viral hepatitis,which would limit their value/applicability in patients with EHS-induced FLF[15].

A PubMed database search (keywords:Liver transplantation,heatstroke) yielded 20 cases of EHS-induced FLF (Figure 2).Of these 20 patients,14 (70%) were treated conservatively and 6 (30%) received OLTx.Among the patients treated conservatively,6 patients (43%) died and 8 (57%) recovered spontaneously.

CONCLUSION

This case series and a review of the literature show that EHS constitute a heterogeneous group of patients observed at emergency departments with varying clinical presentations.Thus,it is difficult to conclusively predict patient outcomes and the urgency of OLTx.Multicenter analysis of existing cases would help to establish more accurate guidelines to manage patients presenting with EHS-induced FLF.

Figure2 Poor contrast enhancement of liver parenchyma in arterial phase in Case 1.