鼠尾草酸對大鼠大腦中動脈阻斷引起的局灶性腦缺血的保護作用

金成 張蘭春 于浩飛

【摘 要】 目的：研究鼠尾草酸預處理對大鼠大腦中動脈阻斷引起的局灶性腦缺血的保護作用。方法：將健康雄性SD大鼠隨機分為7個組：假手術組、模型組、尼莫地平組、燈盞花素組、鼠尾草酸180mg/kg鼠尾草酸90mg/kg組、鼠尾草酸45mg/kg組。各組大鼠連續灌胃給藥7d，末次給藥后，采用改良線栓法制備大鼠大腦中動脈栓塞（Middle Cerebral Artery Occlusion， MCAO）模型，觀察鼠尾草酸預處理對大鼠大腦中動脈阻斷引起的局灶性腦缺血的影響。結果：鼠尾草酸180mg/kg組能明顯縮小腦梗死面積百分比，抑制血小板聚集，顯著性降低纖維蛋白原（FIB）含量;鼠尾草酸90mg/kg組能明顯改善大鼠神經損傷癥狀，縮小腦梗死面積百分比，延長活化部分凝血活酶時間（Activated Partial Thromboplastin Time，ATPP），降低纖維蛋白原（Fibrinogen，FIB）;鼠尾草酸45mg/kg組能延長ATPP，明顯降低纖維蛋白原（FIB）含量。此外，血常規結果顯示，不同劑量鼠尾草酸預處理對MCAO大鼠的的各項生理指標起到一定的調節作用，差異具有統計學意義。結論：鼠尾草酸預處理對大鼠大腦中動脈阻斷引起的局灶性腦缺血的具有良好的保護作用。

【關鍵詞】 鼠尾草酸;MCAO;血小板聚集率;血常規檢測

【中圖分類號】 R285 【文獻標志碼】 A【文章編號】1007-8517（2019）1-0010-07

Abstract：Objective Our aim is to study the role of carnosic acid preconditioning on focal cerebral ischemia caused by middle cerebral artery occlusion in rats.Methods? Healthy male Sprague-Dawley rats were randomly divided into 7 groups： sham operation group， model group， nimodipine group， breviscapine group， carnosic acid 180? mg/kg group， carnosic acid 90 mg/kg group， and carnosic acid 45 mg/kg group.Rats in each group were administered with intragastric administration for 7 days continuously. After the last administration， the Middle Cerebral Artery Occlusion（MCAO）model of rats were prepared by modified suture method to observe the influence of focal cerebral ischemia caused by the treatment of rat middle cerebral artery occlusion. Results? The percentage of cerebral infarction area were significantly reduced， the aggregation of platelets were inhibited， and fibrinogen （FIB） content dramatically was decreased in carnosic acid 180mg/kg group. Then the symptoms of nerve damage in rats were remarkably improved， the percentage of cerebral infarction were reduced， the activated partial thromboplastin time （ATPP）， and decrease fibrinogen （FIB） content were lengthened in carnosic acid 90mg/kg group， compared with model group. Ultimately， the ATPP and fibrinogen （FIB） content of carnosic acid 45mg/kg group were longer and lower than model group. In addition， blood routine test results showed that different doses of carnosic acid pretreatment had certain regulation effects on various physiological indexes of middle cerebral artery occlusion （MCAO） rats， and the difference was statistically significant among the groups. Conclusions? Therefor， the pretreatment of carnosic acid had a good protective effect on focal cerebral ischemia caused by middle cerebral artery occlusion in rats.

Keywords：Carnosic Acid; MCAO; Platelet Aggregation; Blood Routine Test