Galectin—9蛋白過表達對卵巢癌細胞增殖、遷移及凋亡的影響

程亞玉+譚雪瑩+黃飛

[摘要] 目的 探討半乳糖凝集素-9（Galectin-9）蛋白過表達對卵巢癌細胞增殖、遷移和凋亡的影響。 方法 將卵巢癌細胞SKOV-3分為轉染pcDNA3.1-Galectin-9組、pcDNA3.1組和空白對照組，通過Western blot檢測Galectin-9蛋白的表達，CCK-8檢測Galectin-9蛋白對SKOV-3增殖能力的影響，劃痕試驗驗證Galectin-9對SKOV-3遷移能力的作用，Annexin-V-FITC/PI雙染法檢測Galectin-9蛋白對SKOV-3凋亡的影響。 結果 Western blot結果顯示，轉染pcDNA3.1-Galectin-9組Galectin-9蛋白表達明顯高于空白對照組和pcDNA3.1組，差異均有高度統計學意義（均P < 0.01）；CCK-8結果顯示，轉染pcDNA3.1-Galectin-9組增殖能力明顯低于空白對照組和pcDNA3.1組，差異均有高度統計學意義（均P < 0.01），Galectin-9能顯著抑制細胞的增殖能力，并且抑制細胞的增殖能力隨著時間的延長而增強；細胞劃痕試驗結果顯示，轉染pcDNA3.1-Galectin-9組的愈合率明顯高于pcDNA3.1組和空白對照組，差異均有高度統計學意義（均P < 0.01）；流式細胞試驗結果顯示，轉染pcDNA3.1-Galectin-9組的凋亡率明顯高于pcDNA3.1組和空白對照組，差異均有高度統計學意義（均P < 0.01）。 結論 Galectin-9的過表達能夠抑制卵巢癌細胞株的增殖和遷移能力，并促進其凋亡。

[關鍵詞] 半乳糖凝集素-9；卵巢癌；細胞增殖；細胞遷移；細胞凋亡

[中圖分類號] R737.31 [文獻標識碼] A [文章編號] 1673-7210（2018）01（c）-0013-04

[Abstract] Objective To investigate the effects of overexpression of galectin-9 on the proliferation， migration and apoptosis of ovarian cancer cells. Methods The ovarian cancer cells SKOV-3 were divided into pcDNA3.1-galectin-9 transfection group， pcDNA3.1 group and blank control group， Western blot was used to detect the expression of galectin-9 protein， CCK-8 was used to detect the effects of galectin-9 protein for multiplication capacity of SKOV-3， scratch test was used to verify the effects of galectin-9 protein for transfer ability of SKOV-3， Annexin-V-FITC/PI double staining method was used to detect the effects of galectin-9 protein for apoptosis of SKOV-3. Results The results of Western blot showed that the expression of galectin-9 protein in pcDNA3.1-galectin-9 transfection group was significantly higher than those in blank control group and pcDNA3.1 group， the differences were all highly statistically significant （all P < 0.01）. The results of CCK-8 showed that the multiplication capacity of galectin-9 in pcDNA3.1-galectin-9 transfection group was significantly lower than those in blank control group and pcDNA3.1 group， the differences were all highly statistically significant （all P < 0.01）， galectin-9 could significantly inhibit the multiplication capacity of cells， and the capacity of inhibiting proliferation would increase with the extension of time. The results of cell scratch assay showed that the healing rate of pcDNA3.1-galectin-9 transfection group was significantly higher than that of pcDNA3.1 group and blank control group， the differences were all highly statistically significant （all P < 0.01）. The results of flow cytometry showed that the apoptosis rate of pcDNA3.1-galectin-9 transfection group was significantly higher than that of pcDNA3.1 group and blank control group， the differences were highly statistically significant （all P < 0.01）. Conclusion The overexpression of galectin-9 can inhibit the proliferation and migration of ovarian cancer cell lines and promote its apoptosis.endprint