原發(fā)性腎病綜合征患者外周血Th9及IL—9的變化及臨床意義

任金來(lái)等

[摘要] 目的 研究原發(fā)性腎病綜合征（primary nephrotic syndrome，PNS）患者外周血中輔助性T細(xì)胞9（Th9）的含量及其效應(yīng)分子白細(xì)胞介素（IL-9）的表達(dá)情況，探討Th9及IL-9與PNS臨床指標(biāo)的相關(guān)性及可能的免疫學(xué)機(jī)制。 方法 收集35例PNS患者和30名健康對(duì)照者。用流式細(xì)胞儀檢測(cè)PNS患者和健康對(duì)照者外周血中的Th9細(xì)胞在CD4+T細(xì)胞中的比例；酶聯(lián)免疫吸附試驗(yàn)（ELISA）檢測(cè)PNS患者及健康對(duì)照者血漿中IL-9的水平。采用t檢驗(yàn)和Spearman秩相關(guān)分析進(jìn)行統(tǒng)計(jì)學(xué)分析。結(jié)果 PNS患者外周血中Th9細(xì)胞的比例明顯高于健康對(duì)照組差異有統(tǒng)計(jì)學(xué)意義[分別為（1.06±0.20）%，（0.26±0.07）%，P<0.05]。PNS患者外周血IL-9水平高于健康對(duì)照組差異有統(tǒng)計(jì)學(xué)意義[分別為（70.02±13.66） pg/mL，（18.77±5.35） pg/ml，P<0.05]。PNS患者外周血Th9細(xì)胞的表達(dá)與24 h尿蛋白、膽固醇、甘油三酯、尿素氮及IL-9的水平呈正相關(guān)，與血清白蛋白、IgG水平呈負(fù)相關(guān)；PNS患者IL-9的水平與24 h尿蛋白、膽固醇、甘油三酯、尿素氮的水平呈正相關(guān)，與血清白蛋白、IgG、C3水平呈負(fù)相關(guān)。結(jié)論 PNS患者外周血Th9細(xì)胞比例及血漿中l(wèi)L-9水平顯著升高，且與相關(guān)臨床指標(biāo)明顯相關(guān)，提示Th9及IL-9可能在PNS的發(fā)病過(guò)程中發(fā)揮重要作用。

[關(guān)鍵詞] 原發(fā)性腎病綜合征；輔助性T細(xì)胞9；白細(xì)胞介素9

[中圖分類號(hào)] R392.1 [文獻(xiàn)標(biāo)識(shí)碼] A [文章編號(hào)] 1674-0742（2015）06（c）-0006-04

[Abstract] Objective To detect the concentration of T helper cells 9（Th9） and the expression of its effector molecule interleukin（IL-9） in the peripheral blood of primary nephrotic syndrome（PNS） patients， and analyze the relationship of Th9 and IL-9 with the clinical indexes of PNS and their possible role in the immunological pathogenesis of PNS. Methods Thirty-five patients with PNS and 30 healthy controls were included. Th9 cells were obtained from PNS patients and healthy controls and detected by flow cytometry. The levels of IL-9 in PNS patients and healthy controls were detected by ELISA tests. T-test and Spearmans correlation were used for statistical analysis. Results The expression of Th9 in the peripheral blood of PNS patients was significantly higher than normal controls [（1.06±0.20）% vs （0.26±0.07）%]， P<0.05. Meanwhile， the level of IL-9 in the peripheral blood of PNS patients was significantly higher than normal controls [（70.02±13.66） vs （18.77±5.35）]pg/ml， P<0.05. The expression of Th9 was positively correlated with 24-hour urinary protein， cholesterol， triglyceride， blood urea nitrogen， the level of IL-9 respectively， while that of Th9 cells was inversely proportional to serum albumin， immunoglobulin G respectively. The level of IL-9 was positively correlated with 24-hour urinary protein， cholesterol， triglyceride， blood urea nitrogen respectively， and the level of IL-9 was inversely proportional to serum albumin， immunoglobulin G， C3 respectively. Conclusion The expression of Th9 and level of IL-9 in the peripheral blood of PNS patients increase significantly， and which are related with the relative clinical indicators， these indicate that the abnormality of Th9 and IL-9 may play an important role in the pathogenesis of PNS.