中藥復方艾可清加味對艾滋病模型猴不同組織CD4分子表達量的干預作用

朱伯強　符林春　張苗苗

【摘要】 目的：以艾滋病模型猴不同組織的CD4分子表達為靶點，比較艾滋病模型猴不同組織中CD4分子的表達，探討中藥復方艾可清加味對艾滋病模型猴不同組織CD4分子表達的影響。方法：通過標準方法建立艾滋病猴模型，分別設立正常對照組、模型對照組、中藥組和陽性藥物組。正常組不作任何處理，模型組給予生理鹽水灌胃，中藥組給予中藥復方煎劑灌胃，陽性藥物組給予替諾福韋皮下注射治療。治療結束后取材相關組織，用Western blot分析模型猴不同組織的CD4分子的表達。結果：艾滋病模型猴的胃、十二指腸、回腸、結腸、直腸、腸系膜淋巴結、脾、淺表淋巴結織組中CD4表達水平中藥組均數值與模型組比較分別高出11.69%、10.67%、54.55%、15.31%、27.36%、22.68%、2.11%、10.75%。結論：中藥復方可以有效調節艾滋病模型猴中不同組織的免疫情況，提升CD4的表達，改善免疫功能，對猴艾滋病有一定治療作用。

【關鍵詞】 艾滋病；CD4分子；中國恒河猴；免疫印跡；中藥復方

【中圖分類號】R285.5【文獻標志碼】 A【文章編號】1007-8517（2015）10-0007-03

Intervention of traditional Chinese medicine Viagra flavored clear expression of the CD4 molecule on monkeys with AIDS in different tissues

ZHU BoqiangFU Linchun*ZHANG Miaomiao

Tropical Medicine Institute；Guangzhou University of TCM，Guang dong Guang zhou 510405

Abstract： Objective The monkey model of AIDS CD4 molecule expression in different tissues as a target， compare expression monkey model of AIDS CD4 molecules in different tissues to study the effects of traditional Chinese medicine monkey model of AIDS CD4 molecule expression in different tissues. Methods AIDS monkey model by standard methods， namely the establishment of the normal control group， model group， TCM group and positive drug group. Normal group without any treatment， the model group was given normal saline， TCM group were given traditional Chinese medicine decoction， positive drug tenofovir group was given subcutaneous treatment. Minutes after the end of treatmentrelated organizations drawn expression monkey model of CD4 molecules in different tissues analyzed by Western blot. Results AIDS model monkey stomach， duodenum， ileum， colon， rectum， mesenteric lymph nodes， spleen， lymph node tissue superficial group TCM group CD4 expression levels were higher than the value of the model group were 11.69%， 10.67%， 54.55%， 15.31%， 27.36%， 22.68%， 2.11%， 10.75%. [WTHZ]Conclusion[WTBZ] The traditional Chinese medicine can effectively regulate the immune monkey model of AIDS cases in different organizations， to enhance the expression of CD4， improve immune function， AIDS monkeys have some therapeutic effect.

Keywords： AIDS；CD4 molecule；Chinese rhesus；immunoblotting；traditional Chinese medicine

艾滋病（AIDS）全稱為獲得性免疫缺陷綜合征（acquired immunodeficiency syndrome），是人體感染人類免疫缺陷病毒（Human Immunodeficiency Virus，HIV）后，造成CD4+ T淋巴細胞數量進行性減少，引起細胞免疫功能嚴重缺陷，最后導致各種機會性感染、惡性腫瘤及中樞神經系統退行性變的臨床綜合征，嚴重者可導致患者死亡，嚴重威脅人類健康[1-2] 。人類免疫缺陷病毒（HIV）感染人體的機制中， HIV外膜gp120的第1受體是CD4分子，為HIV侵入CD4+T細胞、單核-巨噬細胞等的關鍵。CD4分子與gp120結合后，gp120構象發生變化，然后暴露第2受體CCR5、CXCR4的結合位點，進而激活HI外膜gp41，并插入細胞膜，最終完成病毒包膜與宿主細胞膜的融合[3] 。……