7 Autoimmune Disease

7 Autoimmune Disease

2015044 Exp ression ofm yostatin genem RNA in the m uscle tissue from 75 patients w ith m uscu lar weakness.ZHAO Xiaoping（趙曉萍），et al.Dept Neurol，Beijing Milit General Hosp，Beijing 100700.Chin J Neurol 2014；47（11）：772-775.

Ob jectiveTo investigate myostatin gene mRNA expression in the muscle tissue from patients with muscle weakness suffering from different illness.MethodsThe clinical data of our patients were all from the Muscular Disease Center，Department of Neurology，People's Liberation Army GeneralHospital.A total of75 patients suffering from muscular weakness were included consecutively.Skeletal muscle biopsies were obtained with informed consent from all 75 patients.The diagnosis was confirmed by two senior doctors formuscular disease according to the clinical feature，the results of electromyography，serum creatine kinase activity and histopathological examination.Among them，21 cases were diagnosed as polymyositis，15 cases progressive muscular dystrophy，5 cases neurogenic amyotrophy，4 cases chronic muscle fiber damage，4 casesmitochondrialmyopathy，4 cases lipid storage myopathy，4 cases myotonic dystrophy，3 casesmuscular dystrophy in adults，2 cases dermatomyositis，and 2 cases inclusion bodymyositis.There were 2 cases characterized by pure high activity of creatine kinase.And the other 9 cases were diagnosed as non-neuromuscular disease.The expression ofmyostatin gene mRNA in muscle tissue was evaluated by reverse transcription polymerase chain reaction method，with glyceraldehyde-3-phosphate dehydrogenase as internal reference.ResultsThe expression ofmyostatin genemRNA was detected in 63 patients，but not in other 12 cases，and the percentage of positive expression was84%. The expression index was with great variation，from 0 to 3.52.In positive cohort，the index was correlated posi-tively with the duration of disease（r=0.236，P= 0.041）.The activities of creatine kinase in positive expression cohort were higher than those of negative one，butnot significantly.ConclusionThe expression ofmyostatin genemRNA in muscle tissue may not correlate to the entity of atrophic muscular disease because of its great variation.

（Authors）

2015045 Effect of anticholinesterase agents on acantholysis in a m ouse model of pemphigus vulgaris.WEIGao（韋高），etal.Dept Dermatol，1st Affil Hosp，GuangxiMed Univ，Nanning 530021.Chin JDermatol 2014；47（11）：790-792.

Ob jectiveTo study the effect of anticholinesterase agents on acantholysis in pemphigus vulgaris（PV）by using amousemodel.MethodsFifty-five neonatal BALB/ c mice were divided into four groups：model group injected subcutaneously with the sera of patientswith PV（n= 15），pyridostigmine bromide group（n=15）and neostigminemethylsulfate group（n=15）subcutaneously injected with pyridostigmine bromide and neostigminemethylsulfate respectively，in addition to the sera of PV patients，control group subcutaneously injected with sodium chloride physiological solution（n=10）.The effect of anticholinesterase agents on acantholysis in PV was evaluated in terms of clinical presentation，histopathological manifestations and direct immunofluorescence findings.ResultsThe injection of sera from PV patients induced characteristic changes of PV in neonatal BALB/cmice in themodel group.The degree of acantholysis in themodel group was higher than that in the pyridostigmine bromide group（H=21.584，P＜0.001）and neostigminemethylsulfate group（H=20.641，P＜0.001）.No changes were observed in the control group.ConclusionAnticholinesterase agents can reduce the degree of acantholysis in themousemodel of PV.

（Authors）