4 Disease Caused by Chem ical and Physical Agents

4 Disease Caused by Chem ical and Physical Agents

2015024 Evaluation of the p revention and control measures on coal-burning endem ic fluorosis in Guizhou 2013.ZHANG Boyou（張伯友），et al.Instit Endemic Dis，Guizhou Dis Contr&Prev Center，Guiyang 550004.Chin JEndemiol 2014；33（5）：534-536.

ObjectiveThrough investigation of children fluorosis illness，family households improved stoves and related life styles，to provide a scientific basis for sustainable control of endemic fluorosis.M ethodsIn 2013，in HuishuiCounty and Baiyun District，3 townswere select-ed in each county（district），and 3 villageswere selected in each town.All 8-12 years old children in the school of these villages were checked dental fluorosis，which was diagnosed according to“Dental Fluorosis Diagnosis”（WS/T 208-2011）；at the same time，10 families were selected to survey the situation of improved stoves and related life styles.ResultsDental fluorosis detection rates of 8-12 years old children in Huishui and Baiyun were 2.75%（23/836）and 2.26%（11/487），which were all lower than 30%.Qualified rate of improved stoves and qualified stoves correct utilization rate were all 100.0%（90/90）.For human consumption，the correct rate of corn drying was 100.0%（90/90）；the correct rates of chili drying were 98.9%（89/90）and 100.0%（90/ 90）.ConclusionThe prevention effect is obvious，which has reached the control standards.We should continue to improve the long-term mechanism of comprehensive controlmeasures，and to achieve substantial elimination of coal-burning endemic fluorosis.

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2015025 Effects of trichloroethylene toxicity on normal hum an liver cells and hepatocytes w ith CYP2E1 gene overexpression.XU Xinyun（徐新云），et al. Shenzhen Dis Contr&Prev Center，Shenzhen 518055. Chin J Ind Hyg Occup Dis 2014；32（10）：723-727.

Ob jectiveTo investigate the effects of trichloroethylene（TCE）toxicity on the normal human liver cells（L02 cells）and hepatocyteswith CYP2E1 gene overexpression which was constructed through molecular cloning technology in our laboratory，then to explore the roles of CYP2E1 gene in TCE toxicity.MethodsL02 cells and hepatocytes with CYP2E1 overexpression were treated with various doses of TCE（0，0.25，0.5，1.0，2.0，4.0 mmol/L）for 12 h，the expressions of apoptosis genes（Bcl-2、Caspase-3、Caspase-8、Caspase-9）and oncogenes（c-fos、c-myc、k-ras、p53）were determined by real-time fluorescent PCR.Resu ltsBcl-2 mRNA expression levels increased significantly in normal liver cells and CYP2E1-overexpressing cells after TCE treatment，Bcl-2 levelswere20%～50%higher in CYP2E1-overexpressing cells than in L02 liver cells at doses of 0.25～2.0 mmol/L TCE.Caspase-3，Caspase-8 and caspase-9 mRNA expression increased by 30%～600%in CYP2E1-overexpressing cells at doses of 0.5～4.0 mmol/L TCE when compared with L02 cells（P＜0.01）. Additionally，c-fos、k-ras and c-myc mRNA expression levelswere25%～120%higher in CYP2E1-overexpressing cells than in L02 cells（P＜0.01），p53 mRNA expression levels were lower 10%～50%in CYP2E1-overexpressing cells than in L02 cells（P＜0.05 or P＜0.01）.ConclusionTherewere significantdifferences for apoptosis gene and oncogene expression levels between normal liver cells and CYP2E1-overexpressing cells after they were treated with TCE.These findings indicated that CYP2E1might play an important role in TCEmetabolism in vivo.

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