VEGF—C和VEGFR—3在宮頸增生性病變活檢組織中的表達及其意義

【摘 要】 目的 觀察VEGF-C及其受體VEGFR-3在宮頸增生性病變活檢組織中的表達情況，探討其與宮頸癌的發生、發展及淋巴結轉移的關系，以期成為早期判斷宮頸癌有無淋巴結轉移的重要參考指標。方法 采用免疫組化SP法檢測51例宮頸鱗癌組織活檢中VEGF-C 、VEGFR-3蛋白表達情況，同時采用15例宮頸炎性、修復性增生組織；44例宮頸上皮內瘤變組織（CIN）做為對照。結果 VEGF-C與VEGFR-3在宮頸炎性、修復性增生組織，CIN及宮頸鱗癌活檢組織中的表達依次增高，三者相比，P<0.05，在宮頸鱗癌活檢組織中，VEGF-C與VEGFR-3的表達陽性率淋巴結轉移組明顯高于無淋巴結轉移組（P<0.05）。結論 VEGF-C和VEGFR-3在宮頸鱗癌發生、發展及轉移過程中發揮重要作用，有望作為早期判斷有無淋巴結轉移的重要參考指標之一，為臨床早期干預治療提供可靠實驗室依據。

【關鍵詞】 宮頸增生性病變；活檢組織；VEGF-C；VEGFR-3

【中圖分類號】 R711.74 【文獻標識碼】 BThe Significance and Expression of VEGF-C and VEGFR-3 in Biopsy of Cervical Lesions

Liu Cuiyun Wang Meiling Zhang Chunmei Cheng Hui Chen Weijuan Tian Xiucheng

262700，Department of Pathology，Peopie Hospital of shouguang，Shandong（Liu Cuiyun，Wang Meiling，Chenghui，ChenWeijuan）

262700，Shouguang Hospital for Maternal and Child Health Care，shandong shouguang （Zhang Chunmei）

262700，The Centers for Disease Control and Prevention of shouguang city，Shandong，shouguang（Tian Xiucheng）

【Abstract】 Objective To study the expression of VEGF-C and VEGFR-3 that its receptor in biopsy of cervical lesions，to explore its relationship with occurrence，development and lymph node metastasis of cervical cancer，to become an important reference index for early judgment of cervical cancer with or without lymph node metastasis.Methods Immunohistochemistry was used to test the expression of the VEGF-C and VEGFR-3in 51 cases of cervical squamous cell carcinoma tissue，and at the same time，15 cases of cervical epithelial，inflammmatory and reparative hyperplasia tissue and 44 cases of cervical intraepithelial neoplasia （CIN） tissue were used to be the control.Results The expression of VEGF-C and VEGFR-3 is increasing with the progress of the disease， the difference is significant comparing with eath other （P<0.5）.In the group of cervix squamous carcinoma biopsy tissues with lymph node metastasis，the positive expression rate of VEGF-C and VEGFR-3 was significantly higher than that in the group without lymph node metastasis （P<0.5）.Conclusion VEGF-C and VEGFR-3 plays an important role in occurrence， development and transfer process of cervical squamous cell carcinoma and is expected as an important index in early judgment if there is lymph node metastasis， provides a reliable experimental basis for the clinical treatment of early intervention

【Keywords】 cervical Hyperplasia；biopsy Tissue；VEGF-C；VEGFR-3

宮頸癌在女性生殖道腫瘤中居首位，其病因、發病機理、病程發展及轉移機制目前尚不明確。血管內皮生長因子（VEGF）是血管內皮細胞特異性的肝素結合生長因子，可在體內誘導血管新生，其成員VEGF-C可與其受體VEGFR-3結合，介導淋巴管內皮細胞增殖、分化和淋巴管的增生。近年針對腫瘤抗血管生成的靶向治療已用于臨床并取得較好療效，在腫瘤的發展中淋巴管生成是淋巴轉移的重要途徑，通過對腫瘤淋巴轉移機制的研究表明，阻斷VEGFR-3與VEGF-C 的結合將成為治療腫瘤淋巴轉移的新靶點[1]。本研究通過免疫組化方法檢測宮頸炎性、修復性增生組織，宮頸上皮內瘤變（逐級CIN）及宮頸鱗癌活檢組織中VEGF-C 和VEGFR-3的表達情況。……