摘要:采用乳酸菌(Lactobacillus)發(fā)酵鰱魚(Hypophthaimichthys molitrix)下腳料水解液,通過響應面分析法優(yōu)化其最佳的工藝條件。結(jié)果表明,優(yōu)工藝條件為蔗糖添加量1.80%、乳酸菌接種量1.97%、發(fā)酵溫度42.23 ℃、發(fā)酵時間43.04 h,水解液感觀評價均值能達到92.02分,該水解液具有特殊乳酸發(fā)酵香味和風味,酸度、甜度適中,口感良好。
關(guān)鍵詞:乳酸菌(Lactobacillus);發(fā)酵;鰱魚(Hypophthaimichthys molitrix)下腳料;響應面分析法
中圖分類號:TQ921+.3 文獻標識碼:A 文章編號:0439-8114(2013)17-4175-04
Optimization of Lactobacillus Fermentation of the Hydrolysates of Silver Carp
By-product by Response Surface Methodology
JIANG Sheng-tao1,2,LIN Biao-sheng1,CHEN Li-min1,LAI Xue-lian1
(1.Longyan University,Longyan 364000,F(xiàn)ujian,China; 2.Ningde Normal University,Ningde 352100,F(xiàn)ujian,China)
Abstract: The Lactobacillus fermentation of the hydrolysates of silver carp by-product was studied and the technological conditions were optimized by response surface methodology. The results indicated that the optimal technological conditions were as follows, sugar concentration 1.80%, seeding amount of Lactobacillus 1.97%, fermented for 43.04 h under 42.23 ℃, the mean value of sensory estimate was reached 92.02, the flavor of the fermentation liquid was best with peat-reek of lactic acid, and the liquid tasted sweet and sour, very delicate.
Key words: Lactobacillus; fermentation; silver carp by-product; response surface methodology
收稿日期:2013-01-21
基金項目:福建省教育廳B類龍巖學院校立服務海西項目(JB09209);2012年福建省“大學生創(chuàng)新創(chuàng)業(yè)培訓計劃”項目
作者簡介:江勝滔(1971-),男,福建建甌人,講師,碩士,主要從事微生物發(fā)酵工程研究,(電話)18659774588(電子信箱)windtom-jiang@163.com。
乳酸菌(Lactobacillus)是指能發(fā)酵糖類主要產(chǎn)物為乳酸的一類無芽孢、革蘭氏陽性細菌的總稱。乳酸菌能分解食物中的糖類、蛋白質(zhì),合成維生素,對脂肪類化合物也有微弱的分解能力,能提高脂肪類食物的消化率,促進其消化吸收[1]。
在魚類加工過程中會產(chǎn)生大量的下腳料,包括魚頭、魚尾、魚骨、魚鰭等。據(jù)報道,這些下腳料占魚總重的40%~55%[2]。大量的下腳料不僅造成環(huán)境的污染,而且其營養(yǎng)價值也未得到充分利用,因此必須加強對下腳料有效利用,提高魚的附加利用價值。下腳料用清水洗凈后剪切成小片后用組織搗碎機搗碎,制備成水解液,經(jīng)過酸化處理后可以作為肥料或者飼料添加劑。水解液的制備可以采用硫酸等無機酸,也可以采用乳酸等有機酸。利用有機酸降低酸度具有更大的優(yōu)勢,使用時保藏時間較長、無需中和處理,并且能被土壤利用,可提高飼料的營養(yǎng)價值且不破壞土壤[3]。……