米根霉發酵生產L—乳酸鈣條件的優化

閔捷，于尹尉，肖猛，楊皓，熊海容

摘要：采用米根霉（Rhizopus oryzae） As 3.3462菌株發酵葡萄糖生產乳酸，以CaCO3為中和劑制備L-乳酸鈣，優化種子培養的發酵條件。結果表明，孢子接種量1.8×104個孢子/mL、搖床轉速為200 r/min時所得菌體生長形態較好。發酵培養基初始葡萄糖濃度80 g/L、發酵溫度34 ℃時具有比較高的葡萄糖轉化率。中試結果顯示優化的發酵條件下L-乳酸鈣產率與理論產率非常接近，得到的L-乳酸鈣成品純度高于99%，光學純度達到96%。

關鍵詞：L-乳酸鈣；米根霉（Rhizopus oryzae）；發酵

中圖分類號：TS201.3 文獻標識碼：A 文章編號：0439-8114（2013）05-1141-03

Optimization of L-Lactate Acid Calcium Production Process by Rhizopus oryzae

MIN Jie，YU Yin-wei，XIAO Meng，YANG Hao，XIONG Hai-rong

（College of Life Science， South-Central University for Nationalities， Wuhan 430074， China）

Abstract： Rhizopus oryzae strain As 3.3462 was applied to ferment lactate acid from glucose， and L-lactate acid calcium was produced using CaCO3 as neutralizer. The production process of L-lactate acid calcium was optimized. The results showed that when inoculation dose of spore was 1.8×104 ind. spore/mL and rotation speed at 200 r/min， the growth state of mycelium was well. The optimal fermentation conditions for higher L-lactate calcium were original glucose content in culture medium at 80 g/L and fermentation temperature at 34 ℃. According to pilot test， the productivity yield under the optimized conditions was very close to the theoretical value. The purification of the final L-lactate acid calcium product was higher than 99%； and the optical purity was 96%.

Key words： L-lactate calcium； Rhizopus oryzae； fermentation