昆蟲材料掃描電鏡觀察技術

宋月芹，董鈞鋒，孫會忠

摘要：介紹了昆蟲材料掃描電子顯微鏡觀察的主要技術環節及注意事項。掃描電鏡觀察主要包括材料的預處理、清洗、二重固定、脫水、置換、干燥、粘臺、噴金鍍膜和觀察等步驟。并通過與實例圖片的對比，總結和討論了獲得較好觀察效果的關鍵步驟。該技術具有推廣價值和借鑒意義。

關鍵詞：昆蟲材料；掃描電子顯微鏡；顯微觀察

中圖分類號：Q964 文獻標識碼：A 文章編號：0439-8114（2013）05-1064-02

Scanning Electron Microscope Technology of Insect Material

SONG Yue-qin，DONG Jun-feng，SUN Hui-zhong

（College of Forestry，Henan Sci-Tech University，Luoyang 471003，Henan，China）

Abstract： The main technical aspects and notes of the observation on insect material with scanning electron microscope（SEM） were introduced. The main process of observation by SEM includes pretreatment of the material， double fixation， clean， dehydration， replacement， drying， glue station， spraying metal coating and observation. Through the contrast of experimental pictures， the key technology for obtaining good observation effect was summarized and discussed. The observation technology has wide popularization value and reference significance.

Key words： insect material； scanning electron microscope（SEM）； microscopic observation