Immune regulation of different genotypes of bone marrow mesenchymal stem cells on allogenic islet transplanio

Lin Yujia(林雨佳，2nd Affil Hosp Harbin Med Univ，Harbin 150080，)…∥Chin J Organ Transplant.－2011，32(2).－172 ～176

Immune regulation of different genotypes of bone marrow mesenchymal stem cells on allogenic islet transplanio

Lin Yujia(林雨佳，2nd Affil Hosp Harbin Med Univ，Harbin 150080，)…∥Chin J Organ Transplant.－2011，32(2).－172 ～176

ObjectiveTo compare the immune regulation of syngenic and allogenic mesenchymal stem cells(MSCs)in the transplantation combined with islets.MethodsAfter induction of diabetes in 30 Lewis rats with streptozotocin(STZ)，the recipient Lewis rats received islets from SD rats combined with syngenic(group B)or allogenic(group C)MSCs injection via the portal vein.The group ofislets transplanted alone served as control(group A).The survival time of grafts in all groups was assessed by the level of blood glucose.ELISA was used to detect the levels of interferon－γ(IFN －γ)，interleukin 2(IL－2)，IL－4 and IL－10 in the peripheral blood on the 1st and 3rd day after transplantation.ResultsThe blood glucose levels in all three groups were decreased in a normal range(13.9 mmol/L)and the survival time of grafts in group B(11.38 ±4.03 days)was significantly longer than in group C(5.50 ±2.07 days)as well as group A(2.88 ±1.25 dayS).On the 1st and 3rd day after transplantation，the levels of TH 1 cytokines IFN－γ and IL－2 in group A were significantly higher than those in groups C and B P ＜ 0.05.Meanwhile the levels of TH2 cytokine IL－10 were increased in group B，but there was no significant difference between groups A and C P ＜0.05.The levels of IL －4 had no significant difference among these three groups P＜0.05.ConclusionIslet transplantation combined with MSCs could prolong survival time of grafts.Syngenic MSCs，superior to allogenic ones，are more effective in changing the balance of TH 1/TH2 to TH2.Decreased expression of TH 1 cytokine(IFN － γ，IL－2)，which is closely related to the induction of immune tolerance，is beneficial to the long － term survival of grafts.24 refs，3 figs，3 tabs.

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