鷹嘴豆孢克魯維酵母利用菊芋原料同步糖化與發(fā)酵生產(chǎn)乙醇

俞靜，江佳稀，張永強，呂紅，李育陽，劉建平

復旦大學生命科學學院 遺傳工程國家重點實驗室，上海 200433

鷹嘴豆孢克魯維酵母利用菊芋原料同步糖化與發(fā)酵生產(chǎn)乙醇

俞靜，江佳稀，張永強，呂紅，李育陽，劉建平

復旦大學生命科學學院 遺傳工程國家重點實驗室，上海 200433

菊芋含有大量的菊粉多糖，且種植簡單、產(chǎn)量高，是極具開發(fā)價值的替代玉米等糧食作物生產(chǎn)燃料乙醇的原料。文中研究了鷹嘴豆孢克魯維酵母Y179利用菊芋原料同步糖化與發(fā)酵生產(chǎn)乙醇。鷹嘴豆孢克魯維酵母Y179具有高效分泌菊粉酶的能力，搖瓶試驗顯示Y179酵母能夠利用完全由菊芋原料配制而成的培養(yǎng)基良好生長并發(fā)酵產(chǎn)生乙醇。通氣及溫度對乙醇產(chǎn)量影響明顯，相對厭氧環(huán)境對Y179酵母發(fā)酵產(chǎn)乙醇具有促進作用，30℃發(fā)酵溫度相對37℃和42℃更有利于乙醇產(chǎn)量提高。種子液培養(yǎng)時間及接種量對乙醇產(chǎn)量影響較小。在5 L發(fā)酵罐中以10%(V/V)量接入預培養(yǎng)36 h的Y179種子液，發(fā)酵液完全由菊芋干粉配制而成，總糖含量22%(W/V)，30℃不通氣，300 r/min攪拌，發(fā)酵144 h時，乙醇濃度達到12.3%(V/V)，糖醇轉(zhuǎn)化效率86.9%，糖利用率大于93.6%。初步研究結(jié)果顯示鷹嘴豆孢克魯維酵母Y179在利用菊芋原料生產(chǎn)乙醇方面具有良好應用前景。

菊芋，鷹嘴豆孢克魯維酵母，菊粉酶，同步糖化與發(fā)酵，乙醇發(fā)酵

Abstract:Jerusalem artichoke tubers with inulin as major component are potential feedstock for fuel ethanol production, andKluyveromyces cicerisporusY179 expressing high level of inulinase is suitable for ethanol production with this feedstock by simultaneous saccharification and fermentation approach.In this article, the impact of inoculum, aeration and temperature on ethanol production by the yeast was studied.The experimental results illustrated that inoculum with different levels and seed collected at different cultivation times had negligible effect, while anaerobic conditions enhanced ethanol production, and more ethanol was produced by the yeast at 30°C than at 37°C or 42°C.The medium using Jerusalem artichoke tuber meal as sole component with 22%(W/V)total sugars was inoculated with 36 h-precultured seed at 10%(V/V), and the batch fermentation was conducted in a 5 L fermentor at 30°C with a stirring speed of 300 r/min under anaerobic conditions.After 144 h, 12.3%(V/V)ethanol was produced and the yield of ethanol from sugars was 86.9% of its theoretical one, with 93.6% sugars consumed.These results indicate thatK.

cicerisporusY179 is a promising candidate for industrial ethanol production using Jerusalem artichoke tuber feedstock.

Keywords:Jerusalem artichoke tubers,Kluyveromyces cicerisporus, inulinase, simultaneous saccharification and fermentation,ethanol fermentation

由于石油等化石類資源有限且呈枯竭之勢，能源短缺已是當今社會面臨的一個重大問題。發(fā)展可再生生物質(zhì)能源是解決日趨緊張能源問題的有效途徑，其中燃料乙醇是發(fā)展生物質(zhì)能源的重要選擇。……