水葫蘆內(nèi)生細菌脂肪酸生物標記特性研究

藍江林，劉波，朱育菁，林營志，葛慈斌，劉蕓

福建省農(nóng)業(yè)科學院農(nóng)業(yè)生物資源研究所，福州350003

水葫蘆內(nèi)生細菌脂肪酸生物標記特性研究

藍江林，劉波*，朱育菁，林營志，葛慈斌，劉蕓

福建省農(nóng)業(yè)科學院農(nóng)業(yè)生物資源研究所，福州350003

應用基于微生物細胞脂肪酸成分鑒定的全自動微生物鑒定系統(tǒng)，鑒定水葫蘆內(nèi)生細菌25株，分屬15個屬.共檢測到27個脂肪酸生物標記（PLFAs），這些生物標記分為4種類型，即（1）高頻次分布：在25株細菌中出現(xiàn)13～22次，屬于細菌總體類群（general）的生物標記.（2）中頻次分布：在25株細菌中出現(xiàn)5～7次，可以用于代表細菌屬類群（genus）識別生物標記.（3）低頻次分布：在細菌中的分布概率較小，可以用于指示特定細菌種間差異的生物標記.（4）微頻次分布：僅在一種細菌種類出現(xiàn)，是細菌種（species）特征生物標記.利用脂肪酸生物標記分析同屬細菌不同種的差異，可將微桿菌屬分為2類，第1類包括菌株9Microbacterium barkeri和11Microbacterium imperiale，這兩個菌株 17:0 ISO 和 14：0 ISO 的脂肪酸生物標記含量均為 0.第2類包括 10Microbacterium esteraromaticum、12Microbacterium lacticum和13Microbacterium liquefaciens，這3個菌株均含有17：0 ISO脂肪酸生物標記而區(qū)別于第一類菌株.利用脂肪酸生物標記的差異對25株內(nèi)生細菌進行聚類分析，可將水葫蘆內(nèi)生細菌類群分為4類.不同植物內(nèi)生菌群落中存在著特異的生物標記，利用特征脂肪酸生物標記的分析方法，分析水葫蘆內(nèi)生細菌的群體特性，對于植物內(nèi)生菌微生物群落的研究具有重要意義.

內(nèi)生細菌；脂肪酸生物標記；聚類分析

Received 23 May 2009 accepted 15 July 2009

Abstract：The present paper dealt with isolation and identification of the endophyte bacteria from Eichhornia crassipe（Mart.）Solms.as well as characteristics of their PLFA biomarkers.Results showed that 25 species of endophyte bacteria were identified by means of microbe identification system（MIDI system from USA）.There were 27 PLFA biomarkers detected in 25 species of endophyte bacteria,in which based on the distribution frequency in the bacteria 4 types were clustered e.g.（1）high frequency related to characteristics of microbe in general,（2）middle frequency related to bacteria in general,（3）low frequency related bacterial genus,（4）tiny frequency related to species.According to PLFAs biomarkers, 5speciesofgenusMicrobacterium weredivided into 2 groups,thegroups1 included Microbacterium barkeriand Microbacterium imperiale,there were no biomarker of 17：0 ISO and 14：0 ISO.The groups 2 included Microbacterium esteraromaticum,Microbacterium lacticum and Microbacterium liquefaciens with 17：0 ISO biomarker higher than 1.85%. Using the cluster analysis method the 25 species were classified into 4 groups based on the PLFAs biomarkers.The characteristics of the groups were discussed.There were the specific biomarker represented the endiophyte bacterica which also isolated from other plants.The research provided the analysis pattern to treat the PLFA biomarkers and analyse group characteristics of endophyte bacteria to clear the community structure of endophyte bacteria in Eichhornia crassipe（Mart.）Solms.

Keywords：endophyte bacteria；PLFAs biomarkers；cluster analysis

1 引言（Introduction）

水葫蘆（Eichhornia crassipe（Mart.）Solms）又名鳳眼蓮，屬單子葉多年生植物，雨久花科，鳳……